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Serum steroid profiling of hepatocellular carcinoma associated with hyperadrenocorticism in dogs: A preliminary study
| Title: | Serum steroid profiling of hepatocellular carcinoma associated with hyperadrenocorticism in dogs: A preliminary study |
| Authors: | Oo, Thandar Browse this author | | Sasaki, Noboru Browse this author →KAKEN DB | | Ikenaka, Yoshinori Browse this author | | Ichise, Takahiro Browse this author | | Nagata, Noriyuki Browse this author | | Yokoyama, Nozomu Browse this author | | Sasaoka, Kazuyoshi Browse this author | | Morishita, Keitaro Browse this author | | Nakamura, Kensuke Browse this author →KAKEN DB | | Takiguchi, Mitsuyoshi Browse this author →KAKEN DB |
| Keywords: | adrenocortical hormones | | hepatocarcinogenesis | | baseline serum | | LC/MS/MS | | dogs |
| Issue Date: | 28-Sep-2022 |
| Publisher: | Frontiers Media |
| Journal Title: | Frontiers in veterinary science |
| Volume: | 9 |
| Start Page: | 1014792 |
| Publisher DOI: | 10.3389/fvets.2022.1014792 |
| Abstract: | Background: Hepatocellular carcinoma (HCC) is one of the most common primary liver tumors in humans and dogs. Excessive adrenocortical hormone exposure may cause steroid hepatopathy, which may develop into HCC. In our previous study, hyperadrenocorticism (HAC) was a highly concurrent disease in dogs with HCC. Therefore, this study hypothesized that adrenal steroid alterations might be involved in hepatocarcinogenesis and aimed to specify the relationship between HAC and HCC in dogs. Materials and methods: This study included 46 dogs brought to the Hokkaido University Veterinary Teaching Hospital between March 2019 and December 2020. Owners gave their signed consent for blood collection on their first visit. A total of 19 steroids (14 steroids and 5 metabolites) in the baseline serum of 15 dogs with HCC, 15 dogs with HAC, and 10 dogs with both diseases were quantitatively measured using the developed liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) method. Results: In each group, 11 steroids were detected higher than 50%. The detection rate of steroid hormones did not significantly differ between the groups (p > 0.05). Principle component analysis (PCA) showed that the steroid profiles of the three groups were comparable. Median steroid hormone concentrations were not significantly different between the study diseases (p > 0.05). Conclusion: The developed LC/MS/MS was useful for measuring steroid hormones. Although it was clear that HAC was concurrent in dogs with HCC, none of the serum steroids was suggested to be involved in HCC. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/87312 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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