HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >

Direct MALDI Glycotyping of Glycoproteins toward Practical Subtyping of Biological Samples

Files in This Item:

The file(s) associated with this item can be obtained from the following URL: https://doi.org/10.1021/acsomega.2c05429


Title: Direct MALDI Glycotyping of Glycoproteins toward Practical Subtyping of Biological Samples
Authors: Urakami, Shogo Browse this author
Hinou, Hiroshi Browse this author →KAKEN DB
Issue Date: 1-Nov-2022
Publisher: American Chemical Society
Journal Title: ACS Omega
Volume: 7
Issue: 43
Start Page: 39280
End Page: 39286
Publisher DOI: 10.1021/acsomega.2c05429
Abstract: The rapid analysis of glycan patterns (glycoforms) of glycoproteins can accelerate their quality control and biomarker discovery. We have focused on the direct analysis of glycoprotein glycoforms using matrix-assisted laser desorption/ionization in source decay mass spectrometry (MALDI-ISDMS), called MALDI glycotyping. Our results show that the 1,5-diaminonaphthalene (DAN)/2,5-dihydroxybenzoic acid (DHB)/Na matrix can directly analyze the glycoforms in the femtomolar range of intact glycoproteins. The addition of DAN improved the morphology of the solid matrix due to the mixture of DAN and DHB, which significantly contribute to the high sensitivity of this direct analysis. Adding DAN significantly improved the sensitivity of the glycan precursor ions in the TOF/TOF analysis because of its enhanced fragmentation effect as an efficient UV-MALDI matrix. Further, practical glycoform analysis (glycotyping) of diluted biological samples containing glycoproteins, such as egg whites, was also successfully achieved.
Type: article
URI: http://hdl.handle.net/2115/87399
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 

 - Hokkaido University