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Direct MALDI Glycotyping of Glycoproteins toward Practical Subtyping of Biological Samples
Title: | Direct MALDI Glycotyping of Glycoproteins toward Practical Subtyping of Biological Samples |
Authors: | Urakami, Shogo Browse this author | Hinou, Hiroshi Browse this author →KAKEN DB |
Issue Date: | 1-Nov-2022 |
Publisher: | American Chemical Society |
Journal Title: | ACS Omega |
Volume: | 7 |
Issue: | 43 |
Start Page: | 39280 |
End Page: | 39286 |
Publisher DOI: | 10.1021/acsomega.2c05429 |
Abstract: | The rapid analysis of glycan patterns (glycoforms) of glycoproteins can accelerate their quality control and biomarker discovery. We have focused on the direct analysis of glycoprotein glycoforms using matrix-assisted laser desorption/ionization in source decay mass spectrometry (MALDI-ISDMS), called MALDI glycotyping. Our results show that the 1,5-diaminonaphthalene (DAN)/2,5-dihydroxybenzoic acid (DHB)/Na matrix can directly analyze the glycoforms in the femtomolar range of intact glycoproteins. The addition of DAN improved the morphology of the solid matrix due to the mixture of DAN and DHB, which significantly contribute to the high sensitivity of this direct analysis. Adding DAN significantly improved the sensitivity of the glycan precursor ions in the TOF/TOF analysis because of its enhanced fragmentation effect as an efficient UV-MALDI matrix. Further, practical glycoform analysis (glycotyping) of diluted biological samples containing glycoproteins, such as egg whites, was also successfully achieved. |
Type: | article |
URI: | http://hdl.handle.net/2115/87399 |
Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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