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Generation of viable calves derived from developmentally mature blastocysts produced by on-gel culture

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Title: Generation of viable calves derived from developmentally mature blastocysts produced by on-gel culture
Authors: Saito, Shun Browse this author
Akizawa, Hiroki Browse this author
Furukawa, Eri Browse this author
Yanagawa, Yojiro Browse this author →KAKEN DB
Bai, Hanako Browse this author →KAKEN DB
Takahashi, Masashi Browse this author →KAKEN DB
Kawahara, Manabu Browse this author →KAKEN DB
Keywords: Blastcyst
Embryo transfer
Full-term development
On-gel culture
Issue Date: Oct-2022
Publisher: Society for Reproduction and Development(SRD)
Journal Title: Journal of reproduction and development
Volume: 68
Issue: 5
Start Page: 330
End Page: 334
Publisher DOI: 10.1262/jrd.2022-054
Abstract: Conventional culture systems for bovine embryos are unable to support sustained embryonic development until the developmentally mature blastocyst stage. Although we have previously developed an on-gel culture system that enables bovine blastocysts to complete cell segregation events at day (D) 10 following in vitro culture, the development of D10 blastocysts to term has yet to be achieved. In this study, we attained full-term development of D10 mature blastocysts produced using an on-gel culture system. Two calves derived from on-gel-cultured embryos were vaginally born, showing normal birth and placental weights and no obvious morphological abnormalities. Moreover, we detected no abnormalities in blood metabolic profile analyses. Our findings indicate that on-gel culturing can be used to facilitate the development of developmentally mature blastocysts to term, and produce healthy viable calves. This culture system could make a valuable contribution to cattle production and would enable a of for bovine-specific pre-implantation development.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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