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Generation of viable calves derived from developmentally mature blastocysts produced by on-gel culture
| Title: | Generation of viable calves derived from developmentally mature blastocysts produced by on-gel culture |
| Authors: | Saito, Shun Browse this author | | Akizawa, Hiroki Browse this author | | Furukawa, Eri Browse this author | | Yanagawa, Yojiro Browse this author →KAKEN DB | | Bai, Hanako Browse this author →KAKEN DB | | Takahashi, Masashi Browse this author →KAKEN DB | | Kawahara, Manabu Browse this author →KAKEN DB |
| Keywords: | Blastcyst | | Embryo transfer | | Full-term development | | On-gel culture |
| Issue Date: | Oct-2022 |
| Publisher: | Society for Reproduction and Development（SRD） |
| Journal Title: | Journal of reproduction and development |
| Volume: | 68 |
| Issue: | 5 |
| Start Page: | 330 |
| End Page: | 334 |
| Publisher DOI: | 10.1262/jrd.2022-054 |
| Abstract: | Conventional culture systems for bovine embryos are unable to support sustained embryonic development until the developmentally mature blastocyst stage. Although we have previously developed an on-gel culture system that enables bovine blastocysts to complete cell segregation events at day (D) 10 following in vitro culture, the development of D10 blastocysts to term has yet to be achieved. In this study, we attained full-term development of D10 mature blastocysts produced using an on-gel culture system. Two calves derived from on-gel-cultured embryos were vaginally born, showing normal birth and placental weights and no obvious morphological abnormalities. Moreover, we detected no abnormalities in blood metabolic profile analyses. Our findings indicate that on-gel culturing can be used to facilitate the development of developmentally mature blastocysts to term, and produce healthy viable calves. This culture system could make a valuable contribution to cattle production and would enable a of for bovine-specific pre-implantation development. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/87412 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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