HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Japanese Journal of Veterinary Research >
Volume 52, Number 4 >

Antigenotoxic effect of Pleurotus cornucopiae extracts on the mutagenesis of Salmonella typhimurium TA 98 elicited by benzo[a]pyrene and oxidative DNA lesions in V 79 hamster lung cells

Files in This Item:
163-172.pdf416.92 kBPDFView/Open
Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.52.4.163

Title: Antigenotoxic effect of Pleurotus cornucopiae extracts on the mutagenesis of Salmonella typhimurium TA 98 elicited by benzo[a]pyrene and oxidative DNA lesions in V 79 hamster lung cells
Authors: El. Bohi, Khlood M. Browse this author
Sabik, Laila Browse this author
Muzandu, Kaampwe Browse this author
Shaban, Zein Browse this author
Soliman, Mohamed Browse this author
Ishizuka, Mayumi Browse this author
Kazusaka, Akio Browse this author
Fujita, Shoichi Browse this author
Keywords: Antimutagenesis
Comet assay
CYP1A1
Mushroom
Pleurotus cornucopiae
Issue Date: Feb-2005
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 52
Issue: 4
Start Page: 163
End Page: 172
Abstract: Pleurotus cornucopiae (PC) mushroom with a brilliant yellow pileus is found in the field and known in Japan as Tamogi dake mushroom. The purpose of this paper is to investigate the mechanism of the antimutagenic effect of PC mushroom using both the Ames test and Comet assay. We have found a strong inhibitory effect of both aqueous and organic PC extracts on the mutagenicity elicited by benzo[a]pyrene (B[a]P), This inhibition was dosedependent in reaction mixtures containing cytosolic and microsomal fractions (S-9) from untreated rat liver as well as in those containing S-9 from aryl hydrocarbon receptor (Ah) ligand of Sudan III-treated rats. Sudan III was a potent inducer of cytochrome P4501A (CYP1A) activity.We treated rats with Sudan III to enhance the metabolic activation of B[a]P by the S-9 fraction. To explain whether this antimutagenicity was due to the inhibition of CYP1A activity that metabolically activates B[a]P, we tested the effects of the extracts on activities of CYP1A1 and CYP1A2, represented by ethoxyresorufin Odeethylase (EROD) and methoxyresorufin O-demethylase (MROD) , respectively. Both aqueous and organic extracts inhibited EROD activity at all dose levels, while the inhibitory effect was only observed at high doses with regard to MROD activity. Furthermore, pre-treatment of Chinese hamster V79 cells with PC extracts significantly reduced H202-induced-DNA damage, indicating that PC extracts provide a protective effect against oxidative DNA damage. These results indicate that whole-mushroom extracts contain compounds that may inhibit the metabolic activation of B[a]P by CYP1A1as well as prevent oxidative DNA damage.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/875
Appears in Collections:Japanese Journal of Veterinary Research > Volume 52, Number 4

Submitter: 獣医学部図書室

Export metadata:

OAI-PMH ( junii2 , jpcoar )


 

Feedback - Hokkaido University