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Volume 52, Number 4 >

Lycopene and beta-carotene ameliorate catechol estrogen-mediated DNA damage

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Title: Lycopene and beta-carotene ameliorate catechol estrogen-mediated DNA damage
Authors: Muzandu, Kaampwe Browse this author
El. Bohi, Khlood Browse this author
Shaban, Zein Browse this author
Ishizuka, Mayumi Browse this author
Kazusaka, Akio Browse this author
Fujita, Shoichi Browse this author
Keywords: β-carotene
DNA damage
reactive oxygen species
Issue Date: Feb-2005
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 52
Issue: 4
Start Page: 173
End Page: 184
Abstract: The consumption of fruits and vegetables is associated with a reduced risk of various ailments, including cancer and cardiovascular diseases. Carotenoids, such as lycopene and beta-carotene, are natural constituents of edible plants and may protect against disease. In this study, the influence of lycopene and beta-carotene on DNA damage caused by catechol-estrogens in vitro is examined. One possible mechanism by which catechol estrogens such as 4-hydroxyestradiol (4-OHE2) and 2-hydroxyestradiol, which cause DNA damage in naked plasmid DNA as well as in cells, contributing to the process of carcinogenesis, is through the generation of reactive oxygen species. It was found that both carotenoids at concentrations ranging from 0.25 to 10 μM significantly inhibit strand breakage induced by 4-OHE2/copper sulphate by up to ~90% in plasmid DNA with beta-carotene being slightly more effective. No prooxidant or cytotoxic effects were observed at the concentrations tested. These carotenoids had a similar, though reduced effect on DNA damage as measured by the comet assay, in Chinese hamster lung fibroblasts. The results obtained show that both lycopene and beta-carotene , most probably and mainly through their potent antioxidant properties , are able to inhibit catecholestrogen-mediated DNA damage.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 52, Number 4

Submitter: 獣医学部図書室

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