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Arginase-1 inhibition reduces migration ability and metastatic colonization of colon cancer cells

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Title: Arginase-1 inhibition reduces migration ability and metastatic colonization of colon cancer cells
Authors: Wang, Xiangdong Browse this author
Xiang, Huihui Browse this author
Toyoshima, Yujiro Browse this author
Shen, Weidong Browse this author
Shichi, Shunsuke Browse this author
Nakamoto, Hiroki Browse this author
Kimura, Saori Browse this author
Sugiyama, Ko Browse this author
Homma, Shigenori Browse this author →KAKEN DB
Miyagi, Yohei Browse this author
Taketomi, Akinobu Browse this author →KAKEN DB
Kitamura, Hidemitsu Browse this author →KAKEN DB
Keywords: Arginase 1
Metastatic colonization
Colorectal cancer
Issue Date: 13-Jan-2023
Publisher: BioMed Central
Journal Title: Cancer & Metabolism
Volume: 11
Issue: 1
Start Page: 1
Publisher DOI: 10.1186/s40170-022-00301-z
Abstract: Background: Arginase-1 (ARG1), a urea cycle-related enzyme, catalyzes the hydrolysis of arginine to urea and ornithine, which regulates the proliferation, differentiation, and function of various cells. However, it is unclear whether ARG1 controls the progression and malignant alterations of colon cancer. Methods: We established metastatic colonization mouse model and ARG1 overexpressing murine colon cancer CT26 cells to investigate whether activation of ARG1 was related to malignancy of colon cancer cells in vivo. Living cell numbers and migration ability of CT26 cells were evaluated in the presence of ARG inhibitor in vitro. Results: Inhibition of arginase activity significantly suppressed the proliferation and migration ability of CT26 murine colon cancer cells in vitro. Overexpression of ARG1 in CT26 cells reduced intracellular l-arginine levels, enhanced cell migration, and promoted epithelial-mesenchymal transition. Metastatic colonization of CT26 cells in lung and liver tissues was significantly augmented by ARG1 overexpression in vivo. ARG1 gene expression was higher in the tumor tissues of liver metastasis than those of primary tumor, and arginase inhibition suppressed the migration ability of HCT116 human colon cancer cells. Conclusion: Activation of ARG1 is related to the migration ability and metastatic colonization of colon cancer cells, and blockade of this process may be a novel strategy for controlling cancer malignancy.
Type: article
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 北村 秀光

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