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Hokkaido University Collection of Scholarly and Academic Papers >
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Identification of a Saltol-Independent Salinity Tolerance Polymorphism in Rice Mekong Delta Landraces and Characterization of a Promising Line, Doc Phung
| Title: | Identification of a Saltol-Independent Salinity Tolerance Polymorphism in Rice Mekong Delta Landraces and Characterization of a Promising Line, Doc Phung |
| Authors: | Nguyen, Tam Thanh Browse this author | | Dwiyanti, Maria Stefanie Browse this author | | Sakaguchi, Shuntaro Browse this author | | Koide, Yohei Browse this author →KAKEN DB | | Le, Dung Viet Browse this author | | Watanabe, Toshihiro Browse this author →KAKEN DB | | Kishima, Yuji Browse this author →KAKEN DB |
| Keywords: | Mekong Delta | | Salinity tolerance | | Rice | | Doc Phung | | GWAS (genome-wide association study) | | SES (standard evaluation system) | | Mineral concentrations | | OsPLGG1 |
| Issue Date: | Dec-2022 |
| Publisher: | Springer |
| Journal Title: | Rice |
| Volume: | 15 |
| Issue: | 1 |
| Start Page: | 65 |
| Publisher DOI: | 10.1186/s12284-022-00613-0 |
| Abstract: | The Mekong Delta River in Vietnam is facing salinity intrusion caused by climate change and sea-level rise that is severely affecting rice cultivation. Here, we evaluated salinity responses of 97 rice accessions (79 landraces and 18 improved accessions) from the Mekong Delta population by adding 100 mM NaCl to the nutrient solution for up to 20 days. We observed a wide distribution in salinity tolerance/sensitivity, with two major peaks across the 97 acces-sions when using the standard evaluation system (SES) developed by the International Rice Research Institute. SES scores revealed strong negative correlations (ranging from - 0.68 to - 0.83) with other phenotypic indices, such as shoot elongation length, root elongation length, shoot dry weight, and root dry weight. Mineral concentrations of Na+ in roots, stems, and leaves and Ca2+ in roots and stems were positively correlated with SES scores, suggesting that tolerant accessions lower their cation exchange capacity in the root cell wall. The salinity tolerance of Mekong Delta accessions was independent from the previously described salinity tolerance-related locus Saltol, which encodes an HKT1-type transporter in the salinity-tolerant cultivars Nona Bokra and Pokkali. Indeed, genome-wide association studies using SES scores and shoot dry weight ratios of the 79 accessions as traits identified a single com-mon peak located on chromosome 1. This SNP did not form a linkage group with other nearby SNPs and mapped to the 3 ' untranslated region of gene LOC_Os01g32830, over 6.5 Mb away from the Saltol locus. LOC_Os01g32830 encodes chloroplast glycolate/glycerate translocator 1 (OsPLGG1), which is responsible for photorespiration and growth. SES and shoot dry weight ratios differed significantly between the two possible haplotypes at the causal SNP. Through these analyses, we characterize Doc Phung, one of the most salinity-tolerant varieties in the Mekong Delta population and a promising new genetic resource. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/87742 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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