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Different micro/nano-scale patterns of surface materials influence osteoclastogenesis and actin structure

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Title: Different micro/nano-scale patterns of surface materials influence osteoclastogenesis and actin structure
Authors: Akasaka, Tsukasa Browse this author →KAKEN DB
Tamai, Miho Browse this author →KAKEN DB
Yoshimura, Yoshitaka Browse this author →KAKEN DB
Ushijima, Natsumi Browse this author →KAKEN DB
Numamoto, Shinichiro Browse this author
Yokoyama, Atsuro Browse this author →KAKEN DB
Miyaji, Hirofumi Browse this author →KAKEN DB
Takata, Ryo Browse this author
Yamagata, Shuichi Browse this author →KAKEN DB
Sato, Yoshiaki Browse this author →KAKEN DB
Nakanishi, Ko Browse this author →KAKEN DB
Yoshida, Yasuhiro Browse this author →KAKEN DB
Keywords: micro/nano-pattern
actin structure
resorption pit
Issue Date: 17-Jan-2022
Publisher: Tsinghua university press
Journal Title: Nano research
Volume: 15
Start Page: 4201
End Page: 4211
Publisher DOI: 10.1007/s12274-021-4026-3
Abstract: The surface topography of a material can influence osteoclast activity. However, the surface structural factors that promote osteoclast activity have not yet been investigated in detail. Therefore, we investigated osteoclastogenesis by testing various defined patterns with different dimensions and shapes. The systematic patterns, made of a cyclo-olefin polymer, were prepared at a micron-, submicron-, and nano-scale with a groove, hole, or pillar shape with a 1:1 pitch ratio. RAVV264.7 cells were cultured on these patterns in the presence of the receptor activator of NF-kappa B ligand (RANKL). Osteoclast formation was induced in the order: pillar > groove >= hole. The two-dimensional factors also indicated that submicron-sized patterns strongly induced osteoclast formation. The optimal pillar dimension for osteoclast formation was 500 nm in diameter and 2 mu m in height Furthermore, we observed two types of characteristic actin structure, i.e., belt-like structures with small hollow circles and isolated ring-like structures, which formed on or around the pillars depending on size and height. Furthermore, resorption pits were observed mainly on the top of calcium phosphate-coated pillars. Thus, osteoclasts prefer convex shapes, such as pillars for differentiation and resorption. Our results indicate that osteoclastogenesis can be controlled by designing surfaces with specific morphologies.
Rights: This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at:
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 赤坂 司

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