| Title: | Different micro/nano-scale patterns of surface materials influence osteoclastogenesis and actin structure |
| Authors: | Akasaka, Tsukasa Browse this author →KAKEN DB |
| Tamai, Miho Browse this author →KAKEN DB |
| Yoshimura, Yoshitaka Browse this author →KAKEN DB |
| Ushijima, Natsumi Browse this author →KAKEN DB |
| Numamoto, Shinichiro Browse this author |
| Yokoyama, Atsuro Browse this author →KAKEN DB |
| Miyaji, Hirofumi Browse this author →KAKEN DB |
| Takata, Ryo Browse this author |
| Yamagata, Shuichi Browse this author →KAKEN DB |
| Sato, Yoshiaki Browse this author →KAKEN DB |
| Nakanishi, Ko Browse this author →KAKEN DB |
| Yoshida, Yasuhiro Browse this author →KAKEN DB |
| Keywords: | micro/nano-pattern |
| pillar |
| RAVV264.7 |
| osteoclast |
| actin structure |
| resorption pit |
| Issue Date: | 17-Jan-2022 |
| Publisher: | Tsinghua university press |
| Journal Title: | Nano research |
| Volume: | 15 |
| Start Page: | 4201 |
| End Page: | 4211 |
| Publisher DOI: | 10.1007/s12274-021-4026-3 |
| Abstract: | The surface topography of a material can influence osteoclast activity. However, the surface structural factors that promote osteoclast activity have not yet been investigated in detail. Therefore, we investigated osteoclastogenesis by testing various defined patterns with different dimensions and shapes. The systematic patterns, made of a cyclo-olefin polymer, were prepared at a micron-, submicron-, and nano-scale with a groove, hole, or pillar shape with a 1:1 pitch ratio. RAVV264.7 cells were cultured on these patterns in the presence of the receptor activator of NF-kappa B ligand (RANKL). Osteoclast formation was induced in the order: pillar > groove >= hole. The two-dimensional factors also indicated that submicron-sized patterns strongly induced osteoclast formation. The optimal pillar dimension for osteoclast formation was 500 nm in diameter and 2 mu m in height Furthermore, we observed two types of characteristic actin structure, i.e., belt-like structures with small hollow circles and isolated ring-like structures, which formed on or around the pillars depending on size and height. Furthermore, resorption pits were observed mainly on the top of calcium phosphate-coated pillars. Thus, osteoclasts prefer convex shapes, such as pillars for differentiation and resorption. Our results indicate that osteoclastogenesis can be controlled by designing surfaces with specific morphologies. |
| Rights: | This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s12274-021-4026-3 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/87800 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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