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Isolation and characterization of distinct Rotavirus A in bat and rodent hosts

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Title: Isolation and characterization of distinct Rotavirus A in bat and rodent hosts
Authors: Kishimoto, Mai Browse this author
Kajihara, Masahiro Browse this author →KAKEN DB
Tabata, Koshiro Browse this author
Itakura, Yukari Browse this author
Toba, Shinsuke Browse this author
Ozono, Seiya Browse this author
Sato, Yuko Browse this author
Suzuki, Tadaki Browse this author →KAKEN DB
Ito, Naoto Browse this author →KAKEN DB
Changula, Katendi Browse this author
Qiu, Yongjin Browse this author →KAKEN DB
Mori-Kajihara, Akina Browse this author
Eto, Yoshiki Browse this author
Harima, Hayato Browse this author →KAKEN DB
Mwizabi, Daniel Browse this author
Hang'ombe, Bernard M. Browse this author
Hall, William W. Browse this author
Takada, Ayato Browse this author →KAKEN DB
Orba, Yasuko Browse this author →KAKEN DB
Sawa, Hirofumi Browse this author →KAKEN DB
Sasaki, Michihito Browse this author →KAKEN DB
Keywords: Rotavirus A
Genetic diversity
Glycan specificity
Human intestinal epithelium model
Issue Date: Jan-2023
Publisher: American Society for Microbiology
Journal Title: Journal of virology
Volume: 97
Issue: 1
Start Page: e0145522
Publisher DOI: 10.1128/jvi.01455-22
PMID: 36633410
Abstract: Rotavirus A (RVA) causes diarrheal disease in humans and various animals. Recent studies have identified bat and rodent RVAs with evidence of zoonotic transmission and genome reassortment. However, the virological properties of bat and rodent RVAs with currently identified genotypes still need to be better clarified. Here, we performed virus isolation-based screening for RVA in animal specimens and isolated RVAs (representative strains: 16-06 and MpR12) from Egyptian fruit bat and Natal multimammate mouse collected in Zambia. Whole-genome sequencing and phylogenetic analysis revealed that the genotypes of bat RVA 16-06 were identical to that of RVA BATp39 strain from the Kenyan fruit bat, which has not yet been characterized. Moreover, all segments of rodent RVA MpR12 were highly divergent and assigned to novel genotypes, but RVA MpR12 was phylogenetically closer to bat RVAs than to other rodent RVAs, indicating a unique evolutionary history. We further investigated the virological properties of the isolated RVAs. In brief, we found that 16-06 entered cells by binding to sialic acids on the cell surface, while MpR12 entered in a sialic acid-independent manner. Experimental inoculation of suckling mice with 16-06 and MpR12 revealed that these RVAs are causative agents of diarrhea. Moreover, 16-06 and MpR12 demonstrated an ability to infect and replicate in a 3D-reconstructed primary human intestinal epithelium with comparable efficiency to the human RVA. Taken together, our results detail the unique genetic and virological features of bat and rodent RVAs and demonstrate the need for further investigation of their zoonotic potential. IMPORTANCE Recent advances in nucleotide sequence detection methods have enabled the detection of RVA genomes from various animals. These studies have discovered multiple divergent RVAs and have resulted in proposals for the genetic classification of novel genotypes. However, most of these RVAs have been identified via dsRNA viral genomes and not from infectious viruses, and their virological properties, such as cell/host tropisms, transmissibility, and pathogenicity, are unclear and remain to be clarified. Here, we successfully isolated RVAs with novel genome constellations from three bats and one rodent in Zambia. In addition to whole-genome sequencing, the isolated RVAs were characterized by glycan-binding affinity, pathogenicity in mice, and infectivity to the human gut using a 3D culture of primary intestinal epithelium. Our study reveals the first virological properties of bat and rodent RVAs with high genetic diversity and unique evolutional history and provides basic knowledge to begin estimating the potential of zoonotic transmission.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐々木 道仁

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