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Genetic Analyses of Cell-Free DNA in Pancreatic Juice or Bile for Diagnosing Pancreatic Duct and Biliary Tract Strictures

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Title: Genetic Analyses of Cell-Free DNA in Pancreatic Juice or Bile for Diagnosing Pancreatic Duct and Biliary Tract Strictures
Authors: Nagai, Kosuke Browse this author
Kuwatani, Masaki Browse this author →KAKEN DB
Hirata, Koji Browse this author
Suda, Goki Browse this author
Hirata, Hajime Browse this author
Takishin, Yunosuke Browse this author
Furukawa, Ryutaro Browse this author
Kishi, Kazuma Browse this author
Yonemura, Hiroki Browse this author
Nozawa, Shunichiro Browse this author
Sugiura, Ryo Browse this author
Kawakubo, Kazumichi Browse this author
Sakamoto, Naoya Browse this author →KAKEN DB
Keywords: bile
bile duct
cell-free DNA
liquid biopsy
pancreatic duct
pancreatic juice
Issue Date: 5-Nov-2022
Publisher: MDPI
Journal Title: Diagnostics
Volume: 12
Issue: 11
Start Page: 2704
Publisher DOI: 10.3390/diagnostics12112704
Abstract: Poor prognosis of pancreaticobiliary malignancies is attributed to intrinsic biological aggressiveness and the lack of reliable methods for early diagnosis. This study aimed to evaluate the feasibility and availability of pancreatic juice- and bile-derived cell-free DNA (cfDNA) for diagnosing pancreaticobiliary strictures. From October 2020 to February 2022, pancreatic juice or bile was obtained from 50 patients with pancreaticobiliary strictures during endoscopic retrograde cholangiopancreatography. cfDNAs extracted from the samples were analyzed using next-generation sequencing and a cancer gene panel. The obtained cfDNAs, genetic data and clinical information were analyzed for diagnosis. cfDNA concentrations in pancreatic juice were higher in the intraductal papillary mucinous neoplasm group than in the other groups, whereas those in bile were similar in all groups. In pancreatic juice, the sensitivity, specificity and positive and negative predictive values of cfDNA analyses were 33%, 100%, 100% and 71.4%, respectively, whereas those of cytological analyses were 0%, 100%, 0% and 62.5%, respectively. In bile, those of cell-free DNA analyses were 53%, 75%, 89.5% and 28.6%, respectively, whereas those of cytological analyses were 19%, 100%, 100% and 16%, respectively. In conclusion, pancreatic juice- and bile-derived cfDNA is a novel liquid biopsy tool that can diagnose pancreaticobiliary strictures.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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