Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Environmentally relevant lead (Pb) water concentration induce toxicity in zebrafish (Danio rerio) larvae
This item is licensed under:Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Title: | Environmentally relevant lead (Pb) water concentration induce toxicity in zebrafish (Danio rerio) larvae |
Authors: | Kataba, Andrew Browse this author | Botha, Tarryn L. Browse this author | Nakayama, Shouta M. M. Browse this author →KAKEN DB | Yohannes, Yared B. Browse this author | Ikenaka, Yoshinori Browse this author →KAKEN DB | Wepener, Victor Browse this author | Ishizuka, Mayumi Browse this author →KAKEN DB |
Keywords: | Zebrafish | Lead | Cardiovascular | Apoptosis | Twitching | Fish Embryo Test (FET) |
Issue Date: | 1-Feb-2022 |
Publisher: | Elsevier |
Journal Title: | Comparative biochemistry and physiology Part C: Toxicology & pharmacology |
Volume: | 252 |
Start Page: | 109215 |
Publisher DOI: | 10.1016/j.cbpc.2021.109215 |
Abstract: | Early developmental stages of aquatic organisms including fish are inherently vulnerable to lead (Pb) and other water metal contaminants. However, reports on the deleterious effects of environmentally relevant Pb levels are limited. To this end, we exposed 2.5 h post fertilization (hpf) old zebrafish (Danio rerio) embryos to a range of Pb concentrations encompassing environmentally relevant levels (1, 10, 25, 50 and 100 mu g/L Pb) until 96 hpf. Exposure negatively impacted the development and survival of zebrafish embryos by inducing embryo coagulation related mortalities in a concentration-dependent manner. At 24 hpf, the highest level of exposure (100 mu g/ L Pb) had impaired embryo activity characterized by reduced burst activity and the number of movements per minute made by embryos. At 72 hpf, newly hatched larvae exhibited adverse cardiovascular effects (100 mu g/L Pb group) and neuromuscular effects (50 and 100 mu g/L Pb groups). The antioxidant system dysregulation evidenced by downregulation of catalase, and upregulation of mRNA expression of glutathione S-transferase and cytochrome oxidase subunit I were observed. The pro-apoptotic tumour protein P53 (TP53) and the anti-apoptotic B cell lymphoma-2 (Bcl-2) mRNA expression levels were also affected. The former was downregulated across exposed groups and the latter was upregulated and downregulated in the groups with Pb concentrations less than 50 mu g/L Pb and downregulated in 50 mu g/L Pb, respectively. These findings suggest that Pb within environmentally relevant levels may be deleterious to developing zebrafish. |
Rights: | © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/87844 |
Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 石塚 真由美
|