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Environmentally relevant lead (Pb) water concentration induce toxicity in zebrafish (Danio rerio) larvae

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Title: Environmentally relevant lead (Pb) water concentration induce toxicity in zebrafish (Danio rerio) larvae
Authors: Kataba, Andrew Browse this author
Botha, Tarryn L. Browse this author
Nakayama, Shouta M. M. Browse this author →KAKEN DB
Yohannes, Yared B. Browse this author
Ikenaka, Yoshinori Browse this author →KAKEN DB
Wepener, Victor Browse this author
Ishizuka, Mayumi Browse this author →KAKEN DB
Keywords: Zebrafish
Fish Embryo Test (FET)
Issue Date: 1-Feb-2022
Publisher: Elsevier
Journal Title: Comparative biochemistry and physiology Part C: Toxicology & pharmacology
Volume: 252
Start Page: 109215
Publisher DOI: 10.1016/j.cbpc.2021.109215
Abstract: Early developmental stages of aquatic organisms including fish are inherently vulnerable to lead (Pb) and other water metal contaminants. However, reports on the deleterious effects of environmentally relevant Pb levels are limited. To this end, we exposed 2.5 h post fertilization (hpf) old zebrafish (Danio rerio) embryos to a range of Pb concentrations encompassing environmentally relevant levels (1, 10, 25, 50 and 100 mu g/L Pb) until 96 hpf. Exposure negatively impacted the development and survival of zebrafish embryos by inducing embryo coagulation related mortalities in a concentration-dependent manner. At 24 hpf, the highest level of exposure (100 mu g/ L Pb) had impaired embryo activity characterized by reduced burst activity and the number of movements per minute made by embryos. At 72 hpf, newly hatched larvae exhibited adverse cardiovascular effects (100 mu g/L Pb group) and neuromuscular effects (50 and 100 mu g/L Pb groups). The antioxidant system dysregulation evidenced by downregulation of catalase, and upregulation of mRNA expression of glutathione S-transferase and cytochrome oxidase subunit I were observed. The pro-apoptotic tumour protein P53 (TP53) and the anti-apoptotic B cell lymphoma-2 (Bcl-2) mRNA expression levels were also affected. The former was downregulated across exposed groups and the latter was upregulated and downregulated in the groups with Pb concentrations less than 50 mu g/L Pb and downregulated in 50 mu g/L Pb, respectively. These findings suggest that Pb within environmentally relevant levels may be deleterious to developing zebrafish.
Rights: © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石塚 真由美

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