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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Japanese Journal of Veterinary Research >
Volume 53, Number 1-2 >
Detection of the meq gene in the T cell subsets from chickens infected with Marek's disease virus serotype 1
| Title: | Detection of the meq gene in the T cell subsets from chickens infected with Marek's disease virus serotype 1 |
| Authors: | Chang, Kyung-Soo Browse this author | | Ohashi, Kazuhiko Browse this author | | Lee, Sung-Il Browse this author | | Takagi, Michihiro Browse this author | | Onuma, Misao Browse this author |
| Keywords: | Marek’s disease virus | | meq | | L-meq | | CD4+ T cell | | CD8+ T cell |
| Issue Date: | Aug-2005 |
| Publisher: | The Graduate School of Veterinary Medicine, Hokkaido University |
| Journal Title: | Japanese Journal of Veterinary Research |
| Volume: | 53 |
| Issue: | 1-2 |
| Start Page: | 3 |
| End Page: | 11 |
| Abstract: | The meq gene was thought to be only detected in Marek's disease virus serotype 1 (MDV1) including a very virulent strain, Md5, while L-meq, in which a 180-bp sequence is inserted into the meq open reading frame, is found in other strains of MDV1, such as CVI988/R6. However, both meq and L-meq were previously detected by PCR in chickens infected with MDV1，suggesting hat MDV1 may consists of at least two subpopulations, one with meq, the other with L-meq. To further analyze these subpopulations, we analyzed the time course changes in distribution of these subpopulations among T cell subsets from chickens infected with MDV1. Both meq and L-meq were detected in CD4+ and CD8+ T cells infected with strain Md5 or CVI988/R6. The shift in MDV subpopulations from one displaying meq to the other displaying L-meq and/or the conversion from meq to L-meq occurred mainly in the CD8+ T cell subset from Md5- infected chickens. PCR products corresponding to L-meq rather than meq were frequently amplified from the CD8+ T cell subset from CVI988/R6-infected chickens. These results suggest that a dominant subpopulation of MDV1 changes depending on the T cell subsets, and that L-meq is dominantly present in the CD8+ T cells which play a role in the clearance of pathogenic agents. |
| Type: | bulletin (article) |
| URI: | http://hdl.handle.net/2115/879 |
| Appears in Collections: | Japanese Journal of Veterinary Research > Volume 53, Number 1-2
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Submitter: 獣医学部図書室
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