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Detection of the meq gene in the T cell subsets from chickens infected with Marek's disease virus serotype 1

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.53.1-2.3

Title: Detection of the meq gene in the T cell subsets from chickens infected with Marek's disease virus serotype 1
Authors: Chang, Kyung-Soo Browse this author
Ohashi, Kazuhiko Browse this author
Lee, Sung-Il Browse this author
Takagi, Michihiro Browse this author
Onuma, Misao Browse this author
Keywords: Marek’s disease virus
meq
L-meq
CD4+ T cell
CD8+ T cell
Issue Date: Aug-2005
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 53
Issue: 1-2
Start Page: 3
End Page: 11
Abstract: The meq gene was thought to be only detected in Marek's disease virus serotype 1 (MDV1) including a very virulent strain, Md5, while L-meq, in which a 180-bp sequence is inserted into the meq open reading frame, is found in other strains of MDV1, such as CVI988/R6. However, both meq and L-meq were previously detected by PCR in chickens infected with MDV1,suggesting hat MDV1 may consists of at least two subpopulations, one with meq, the other with L-meq. To further analyze these subpopulations, we analyzed the time course changes in distribution of these subpopulations among T cell subsets from chickens infected with MDV1. Both meq and L-meq were detected in CD4+ and CD8+ T cells infected with strain Md5 or CVI988/R6. The shift in MDV subpopulations from one displaying meq to the other displaying L-meq and/or the conversion from meq to L-meq occurred mainly in the CD8+ T cell subset from Md5- infected chickens. PCR products corresponding to L-meq rather than meq were frequently amplified from the CD8+ T cell subset from CVI988/R6-infected chickens. These results suggest that a dominant subpopulation of MDV1 changes depending on the T cell subsets, and that L-meq is dominantly present in the CD8+ T cells which play a role in the clearance of pathogenic agents.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/879
Appears in Collections:Japanese Journal of Veterinary Research > Volume 53, Number 1-2

Submitter: 獣医学部図書室

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