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Upregulation of renal renin-angiotensin system in mouse diabetic nephropathy

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Title: Upregulation of renal renin-angiotensin system in mouse diabetic nephropathy
Authors: Tamura, Jun Browse this author
Konno, Akihiro Browse this author
Hashimoto, Yoshiharu Browse this author →KAKEN DB
Kon, Yasuhiro Browse this author →KAKEN DB
Keywords: diabetes
renin-angiotensin system
Issue Date: Aug-2005
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 53
Issue: 1-2
Start Page: 13
End Page: 26
Abstract: The aim of this study was to clarify the role of the renal renin-angiotensin system (RAS) in diabetic nephropathy (DN) , which was induced by injection of streptozotocin (STZ). Male CBA/N and CBA/J mice were compared in this study. The former possesses a single renin gene, Ren1. whereas the latter carries two renin genes, Ren1 and Ren2. To examine the molecular dynamics of renal RAS, including renin, angiotensinogen (Agt). angiotensin-converting enzyme (Ace). angiotensin type 1 (Agtr1) and type 2 (Agtr2) receptors in experimental DN, we performed laser-microdissection (LMD) followed by reverse transcriptase nested polymerase chain reaction using each specific primer pairs and immunohistochemistry for renin and angiotensin II. CBA/N mice had a higher response after injection of STZ than CBA/J mice, showing a significant increase of the kidney/body weight ratio, although there was no significant difference between the two strains for the blood glucose level or pancreatic β-cell response. The onset of renal pathological changes associated with DN was earlier and more severe in CBA/N mice than in CBA/J mice. Distinct immunoreactivities for renin and angiotensin. These findings suggest that intrarenal RAS plays an important role in the onset of renal pathological changes associated with DN. Additionally, Ren 1 mice have more severe histopathological nephropathy than Ren1 Ren2 mice, followed by marked production of angiotensin II.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 53, Number 1-2

Submitter: 獣医学部図書室

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