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Volume 53, Number 1-2 >

Cloning and characterization of cDNA encoding a prohibitin-like protein from Theileria orientalis

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.53.1-2.27

Title: Cloning and characterization of cDNA encoding a prohibitin-like protein from Theileria orientalis
Authors: He, Weiyong Browse this author
Ohashi, Kazuhiko Browse this author
Sugimoto, Chihiro Browse this author →KAKEN DB
Tsuji, Masayoshi Browse this author
Onuma, Misao Browse this author
Keywords: Theileria orientalis
prohibitin
piroplasm
Issue Date: Aug-2005
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 53
Issue: 1-2
Start Page: 27
End Page: 35
Abstract: A cDNA clone encoding a prohibitin-like protein (Toprh) was isolated from a piroplasm cDNA library of Theileria orientalis and its nucleotide sequence was determined. An open reading frame, encoding a polypeptide of 278 amino acid residues, was found in Toprh cDNA sequence. An intron of 89 bp was identified when this cDNA clone was compared with the Toprh gene in the genome of T. orientalis. The deduced amino acid sequence of Toprh shares 93.8, 93.1 and 69.1% identities with the prohibitins of T. parva (from chromosome1), T. annulata (from chromosome 1), and Plasmodium falciparum, (from chromosome 10), respectively. By Western blot analysis, Toprh was found to be expressed in the piroplasm stage of the parasites.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/881
Appears in Collections:Japanese Journal of Veterinary Research > Volume 53, Number 1-2

Submitter: 獣医学部図書室

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