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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Consumption of indigestible saccharides and administration of Bifidobacterium pseudolongum reduce mucosal serotonin in murine colonic mucosa
| Title: | Consumption of indigestible saccharides and administration of Bifidobacterium pseudolongum reduce mucosal serotonin in murine colonic mucosa |
| Authors: | Tatsuoka, Misa Browse this author | | Osaki, Yosuke Browse this author | | Ohsaka, Fumina Browse this author →KAKEN DB | | Tsuruta, Takeshi Browse this author | | Kadota, Yoshihiro Browse this author | | Tochio, Takumi Browse this author | | Hino, Shingo Browse this author | | Morita, Tatsuya Browse this author | | Sonoyama, Kei Browse this author →KAKEN DB |
| Keywords: | Serotonin | | 1-kestose | | Bifidobacteria | | Gut microbiota | | SCFA |
| Issue Date: | 28-Feb-2022 |
| Publisher: | Cambridge University Press |
| Journal Title: | British journal of nutrition |
| Volume: | 127 |
| Issue: | 4 |
| Start Page: | 513 |
| End Page: | 525 |
| Publisher DOI: | 10.1017/S0007114521001306 |
| Abstract: | SCFA increase serotonin (5-hydroxytryptamine, 5-HT) synthesis and content in the colon in vitro and ex vivo, but little is known in vivo. We tested whether dietary indigestible saccharides, utilised as a substrate to produce SCFA by gut microbiota, would increase colonic 5-HT content in mice. Male C57BL/6J mice were fed a purified diet and water supplemented with 4 % (w/v) 1-kestose (KES) for 2 weeks. Colonic 5-HT content and enterochromaffin (EC) cell numbers were lower in mice supplemented with KES than those without supplementation, while monoamine oxidase A activity and mRNA levels of tryptophan hydroxylase 1 (Tph1), chromogranin A (Chga), Slc6a4 and monoamine oxidase A (Maoa) genes in the colonic mucosa, serum 5-HT concentration and total 5-HT content in the colonic contents did not differ between groups. Caecal acetate concentration and Bifidobacterium pseudolongum population were higher in KES-supplemented mice. Similar trends were observed in mice supplemented with other indigestible saccharides, that is, fructo-oligosaccharides, inulin and raffinose. Intragastric administration of live B. pseudolongum (10(8) colony-forming units/d) for 2 weeks reduced colonic 5-HT content and EC cell numbers. These results suggest that changes in synthesis, reuptake, catabolism and overflow of 5-HT in the colonic mucosa are not involved in the reduction of colonic 5-HT content by dietary indigestible saccharides in mice. We propose that gut microbes including B. pseudolongum could contribute to the reduction of 5-HT content in the colonic mucosa via diminishing EC cells. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/88134 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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