| Title: | Lipid droplet accumulation and adipophilin expression in follicular thyroid carcinoma |
| Authors: | Hayakawa, Michiyo Browse this author |
| Taylor, J. Nicholas Browse this author |
| Nakao, Ryuta Browse this author |
| Mochizuki, Kentaro Browse this author |
| Sawai, Yuki Browse this author |
| Hashimoto, Kosuke Browse this author |
| Tabata, Koji Browse this author |
| Kumamoto, Yasuaki Browse this author |
| Fujita, Katsumasa Browse this author |
| Konishi, Eiichi Browse this author |
| Hirano, Shigeru Browse this author |
| Tanaka, Hideo Browse this author |
| Komatsuzaki, Tamiki Browse this author →KAKEN DB |
| Harada, Yoshinori Browse this author |
| Keywords: | Follicular neoplasm |
| Follicular thyroid carcinoma |
| Follicular thyroid adenoma |
| Lipid droplet |
| Adipophilin |
| Issue Date: | 15-Jan-2023 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and biophysical research communications |
| Volume: | 640 |
| Start Page: | 192 |
| End Page: | 201 |
| Publisher DOI: | 10.1016/j.bbrc.2022.12.007 |
| Abstract: | Follicular neoplasms of the thyroid include follicular thyroid carcinoma (FTC) and follicular thyroid adenoma (FTA). However, the differences in cytological findings between FTC and FTA remain undetermined. Here, we aimed to evaluate the accumulation of lipid droplets (LDs) and the expression of adipophilin (perilipin 2/ADRP/ADFP), a known LD marker, in cultured FTC cells. We also immunohistochemically compared adipophilin expression in the FTC and FTA of resected human thyroid tissues. Cultured FTC (FTC-133 and RO82W-1) possessed increased populations of LDs compared to thyroid follicular epithelial (Nthy-ori 3-1) cells. In vitro treatment with phosphatidylinositol-3-kinase (PI3K)/Akt/ mammalian target of rapamycin (mTOR) signaling inhibitors (LY294002, MK2206, and rapamycin) in FTC-133 cells downregulated the PI3K/Akt/mTOR/sterol regulatory element-binding protein 1 (SREBP1) signaling pathway, resulting in a significant reduction in LD accumulation. SREBP1 is a master transcription factor that controls lipid metabolism. Fluorescence immunocytochemistry revealed adipophilin expression in the LDs of FTC-133 cells. Immunohistochemical analysis of surgically resected human thyroid tissues revealed significantly increased expression of adipophilin in FTC compared with FTA and adjacent non-tumorous thyroid epithelia. Taken together, LDs and adipophilin were abundant in cultured FTC; the evaluation of adipophilin expression can help distinguish FTC from FTA in surgical specimens. (c) 2022 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Type: | article |
| URI: | http://hdl.handle.net/2115/88150 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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