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Asymbiotic mass production of the arbuscular mycorrhizal fungus Rhizophagus clarus
| Title: | Asymbiotic mass production of the arbuscular mycorrhizal fungus Rhizophagus clarus |
| Authors: | Tanaka, Sachiko Browse this author | | Hashimoto, Kayo Browse this author | | Kobayashi, Yuuki Browse this author | | Yano, Koji Browse this author | | Maeda, Taro Browse this author | | Kameoka, Hiromu Browse this author | | Ezawa, Tatsuhiro Browse this author →KAKEN DB | | Saito, Katsuharu Browse this author | | Akiyama, Kohki Browse this author | | Kawaguchi, Masayoshi Browse this author |
| Issue Date: | 12-Jan-2022 |
| Publisher: | Nature Portfolio |
| Journal Title: | Communications Biology |
| Volume: | 5 |
| Issue: | 1 |
| Start Page: | 43 (2022) |
| Publisher DOI: | 10.1038/s42003-021-02967-5 |
| Abstract: | Arbuscular mycorrhizal (AM) symbiosis is a mutually beneficial interaction between fungi and land plants and promotes global phosphate cycling in terrestrial ecosystems. AM fungi are recognised as obligate symbionts that require root colonisation to complete a life cycle involving the production of propagules, asexual spores. Recently, it has been shown that Rhizophagus irregularis can produce infection-competent secondary spores asymbiotically by adding a fatty acid, palmitoleic acid. Furthermore, asymbiotic growth can be supported using myristate as a carbon and energy source for their asymbiotic growth to increase fungal biomass. However, the spore production and the ability of these spores to colonise host roots were still limited compared to the co-culture of the fungus with plant roots. Here we show that a combination of two plant hormones, strigolactone and jasmonate, induces the production of a large number of infection-competent spores in asymbiotic cultures of Rhizophagus clarus HR1 in the presence of myristate and organic nitrogen. Inoculation of asymbiotically-generated spores promoted the growth of host plants, as observed for spores produced by symbiotic culture system. Our findings provide a foundation for the elucidation of hormonal control of the fungal life cycle and the development of inoculum production schemes. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/88195 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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