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Volume 53, Number 1-2 >

Identification of heat shock protein 70 in canine reticulocytes and mature erythrocytes

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タイトル: Identification of heat shock protein 70 in canine reticulocytes and mature erythrocytes
著者: Jeong, Ja-Ryong 著作を一覧する
Yamasaki, Masahiro 著作を一覧する
Komatsu, Tomohiko 著作を一覧する
Inaba, Mutsumi 著作を一覧する
Yamato, Osamu 著作を一覧する
Maede, Yoshimitsu 著作を一覧する
キーワード: canine Hsp 70
erythrocyte maturation
reticulocyte lysate
発行日: 2005年 8月
出版者: The Graduate School of Veterinary Medicine, Hokkaido University
誌名: Japanese Journal of Veterinary Research
巻: 53
号: 1-2
開始ページ: 37
終了ページ: 46
抄録: In the present study, we demonstrated that heat shock protein 70 (Hsp70) was present in both canine reticulocytes and mature erythrocytes, and that the canine Hsp 70 in reticulocytes was decreased along with the maturation of the cells into erythrocytes. These results suggest that the Hsp 70 in canine reticulocytes might act as a chaperone to remove unnecessary proteins during reticulocyte maturation. We also demonstrated that Hsp 70 was present in exosomes from reticulocytes during their maturation in in vitro culture. Furthermore, the concentration of Hsp 70 in reticulocyte membranes was increased in proportion to an increase of the protein in exosomes until 48 hours after the incubation of reticulocytes in vitro. At 96 hours of the incubation, however, only a trace amount of Hsp 70 was detected in the membrane, while a large amount of the protein was present in the exosomes. These results suggest that Hsp 70 in canine reticulocytes might play an important role for exosome formation in reticulocytes, resulting in the maturation of the cells.
資料タイプ: bulletin
出現コレクション:Volume 53, Number 1-2


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