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Hokkaido University Collection of Scholarly and Academic Papers >
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Mammaglobin 1 mediates progression of trastuzumab-resistant breast cancer cells through regulation of cyclins and NF-κB
| Title: | Mammaglobin 1 mediates progression of trastuzumab-resistant breast cancer cells through regulation of cyclins and NF-κB |
| Authors: | Kusumastuti, Ratih Browse this author | | Kumagai, Yuji Browse this author | | Ishihara, Seiichiro Browse this author →KAKEN DB | | Enomoto, Atsushi Browse this author →KAKEN DB | | Murakami, Takashi Browse this author →KAKEN DB | | Yasuda, Motoaki Browse this author →KAKEN DB | | Haga, Hisashi Browse this author →KAKEN DB |
| Keywords: | cyclin | | invasion | | MGB1 | | migration | | resistance | | trastuzumab |
| Issue Date: | 29-Jul-2022 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Febs open bio |
| Volume: | 12 |
| Issue: | 10 |
| Start Page: | 1797 |
| End Page: | 1813 |
| Publisher DOI: | 10.1002/2211-5463.13468 |
| Abstract: | Overexpression of human epidermal growth factor receptor 2 (HER2) in various cancers is correlated with poor patient survival. Trastuzumab, a recombinant humanized monoclonal antibody against HER2, has been considered to be a first-line therapy for HER2-positive breast cancer patients, but its usefulness is limited by the development of resistance. In this study, we established resistant cells by long-term treatment with trastuzumab. These cells showed higher proliferation, invasion, and migration abilities than the wild-type cells. Mammaglobin 1 (MGB1), cyclin D1, E1, A2, and phosphorylated NF-κB (p-p65) were upregulated in resistant cells. These proteins regulate cell proliferation, migration, and invasion of resistant cells. Depletion of MGB1 decreased cyclin and p-p65 expression. Cyclin D1 and A2, but not E1 expression, were affected by p-p65 downregulation. In summary, our results indicate that MGB1 expression is increased in breast cancer cells that have gained resistance to trastuzumab, and suggest that MGB1 promotes aggressiveness through cyclin and NF-κB regulation. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/88243 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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