Impact of membrane protein-lipid interactions on formation of bilayer lipid membranes on SAM-modified gold electrode

Kato, Masaru; Masuda, Yuya; Yoshida, Narumi; Tosha, Takehiko; Shiro, Yoshitsugu; Yagi, Ichizo

doi:10.1016/j.electacta.2021.137888


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Impact of membrane protein-lipid interactions on formation of bilayer lipid membranes on SAM-modified gold electrode

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Title:	Impact of membrane protein-lipid interactions on formation of bilayer lipid membranes on SAM-modified gold electrode
Authors:	Kato, Masaru Browse this author →KAKEN DB
	Masuda, Yuya Browse this author
	Yoshida, Narumi Browse this author
	Tosha, Takehiko Browse this author →KAKEN DB
	Shiro, Yoshitsugu Browse this author →KAKEN DB
	Yagi, Ichizo Browse this author →KAKEN DB
Keywords:	Membrane protein
	Surface-enhanced infrared absorption spectroscopy
	Nitric oxide reductase
	Protein-tethered bilayer lipid membrane
	Protein film electrochemistry
Issue Date:	20-Mar-2021
Journal Title:	Electrochimica Acta
Volume:	373
Start Page:	137888
Publisher DOI:	10.1016/j.electacta.2021.137888
Abstract:	In protein film electrochemistry for redox-active membrane proteins, the reconstitution of protein-tethered bilayer lipid membranes (ptBLMs) can maximize their functionality and stability at electrolyte/electrode interfaces. To understand the impact of protein–lipid interactions on the ptBLM formation, we tracked the reconstitution process of a lipid bilayer in the presence or the absence of a transmembrane enzyme of cytochrome c-dependent nitric oxide reductase (cNOR) on a mixed self-assembled monolayer (SAM)/Au electrode. Time-resolved surface-enhanced infrared absorption (SEIRA) spectroscopy and electrochemical measurements revealed that the protein–lipid interaction affected the formation kinetics for the phospholipid bilayer and the electrocatalytic stability of the cNOR-modified electrode but not the fluidity of the phospholipid bilayer.
Rights:	© 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Rights:	http://creativecommons.org/licenses/by-nc-nd/4.0/
Type:	article (author version)
URI:	http://hdl.handle.net/2115/88526
Appears in Collections:	環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 加藤優

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