HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
International Institute for Zoonosis Control >
Peer-reviewed Journal Articles, etc >

Molecular Characterization of Mutations in Isoniazid- and Rifampicin-Resistant Mycobacterium tuberculosis Isolated in Thailand

Files in This Item:

The file(s) associated with this item can be obtained from the following URL:

Title: Molecular Characterization of Mutations in Isoniazid- and Rifampicin-Resistant Mycobacterium tuberculosis Isolated in Thailand
Authors: Rudeeaneksin, Janisara Browse this author
Phetsuksiri, Benjawan Browse this author
Nakajima, Chie Browse this author →KAKEN DB
Fukushima, Yukari Browse this author
Suthachai, Worasak Browse this author
Tipkrua, Nattakan Browse this author
Suthum, Krairerk Browse this author
Jekloh, Nasron Browse this author
Bunchoo, Supranee Browse this author
Srisungngam, Sopa Browse this author
Klayut, Wiphat Browse this author
Hamada, Shigeyuki Browse this author
Suzuki, Yasuhiko Browse this author →KAKEN DB
Keywords: Tuberculosis
Issue Date: 24-Jan-2023
Publisher: 国立感染症研究所(National Institute of Infectious Diseases, Japan)
Journal Title: Japanese journal of infectious diseases
Volume: 76
Issue: 1
Start Page: 39
End Page: 45
Publisher DOI: 10.7883/yoken.JJID.2022.055
Abstract: The control of drug-resistant tuberculosis (TB) is a major challenge. The frequency and mutation characteristics indicate the efficiency of molecular tests for the rapid detection of TB drug resistance. This study examined the existence of katG and inhA mutations for isoniazid (INH) resistance and rpoB mutations for rifampicin (RFP) resistance. In total, 178 drug-resistant Mycobacterium tuberculosis (MTB) isolates were analyzed. Mutations in katG encoding and inhA regulatory regions were detected in 136/168 (81.0%) and 29/168 (17.3%), respectively, with the most prominent mutation of Ser315Thr substitution in katG in 126/168 (75.0%), and-15 C to T substitution in the regulatory region of the inhA (26/168; 15.5%). Two distinct katG mutations (Tyr337Cys, 1003InsG) were identified. Of 125 RFP-resistant isolates, 118 (94.4%) carried mutations affecting the 81-bp RFP resistance-determining region, with the most commonly affected codons 450, 445, and 435 identified in 74 (59.2%), 26 (20.8%), and 12 (9.6%) isolates, respectively. Genetic mutations were highly associated with phenotypic INH and RFP resistance, and the majority shared similarities with those reported in previous studies in Thailand and other Asian countries. These data are useful for guiding the use and improvement of molecular tests for TB drug resistance.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University