Title: | Molecular Characterization of Mutations in Isoniazid- and Rifampicin-Resistant Mycobacterium tuberculosis Isolated in Thailand |
Authors: | Rudeeaneksin, Janisara Browse this author |
Phetsuksiri, Benjawan Browse this author |
Nakajima, Chie Browse this author →KAKEN DB |
Fukushima, Yukari Browse this author |
Suthachai, Worasak Browse this author |
Tipkrua, Nattakan Browse this author |
Suthum, Krairerk Browse this author |
Jekloh, Nasron Browse this author |
Bunchoo, Supranee Browse this author |
Srisungngam, Sopa Browse this author |
Klayut, Wiphat Browse this author |
Hamada, Shigeyuki Browse this author |
Suzuki, Yasuhiko Browse this author →KAKEN DB |
Keywords: | Tuberculosis |
rifampicin-resistance |
isoniazid-resistance |
Thailand |
Issue Date: | 24-Jan-2023 |
Publisher: | 国立感染症研究所(National Institute of Infectious Diseases, Japan) |
Journal Title: | Japanese journal of infectious diseases |
Volume: | 76 |
Issue: | 1 |
Start Page: | 39 |
End Page: | 45 |
Publisher DOI: | 10.7883/yoken.JJID.2022.055 |
Abstract: | The control of drug-resistant tuberculosis (TB) is a major challenge. The frequency and mutation characteristics indicate the efficiency of molecular tests for the rapid detection of TB drug resistance. This study examined the existence of katG and inhA mutations for isoniazid (INH) resistance and rpoB mutations for rifampicin (RFP) resistance. In total, 178 drug-resistant Mycobacterium tuberculosis (MTB) isolates were analyzed. Mutations in katG encoding and inhA regulatory regions were detected in 136/168 (81.0%) and 29/168 (17.3%), respectively, with the most prominent mutation of Ser315Thr substitution in katG in 126/168 (75.0%), and-15 C to T substitution in the regulatory region of the inhA (26/168; 15.5%). Two distinct katG mutations (Tyr337Cys, 1003InsG) were identified. Of 125 RFP-resistant isolates, 118 (94.4%) carried mutations affecting the 81-bp RFP resistance-determining region, with the most commonly affected codons 450, 445, and 435 identified in 74 (59.2%), 26 (20.8%), and 12 (9.6%) isolates, respectively. Genetic mutations were highly associated with phenotypic INH and RFP resistance, and the majority shared similarities with those reported in previous studies in Thailand and other Asian countries. These data are useful for guiding the use and improvement of molecular tests for TB drug resistance. |
Type: | article |
URI: | http://hdl.handle.net/2115/88640 |
Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|