Title: | Transcriptional dynamics of granulocytes in direct response to incubation with SARS-CoV-2 |
Authors: | Nakazawa, Daigo Browse this author →KAKEN DB |
Takeda, Yohei Browse this author |
Kanda, Masatoshi Browse this author |
Tomaru, Utano Browse this author |
Ogawa, Haruko Browse this author |
Kudo, Takashi Browse this author |
Shiratori-Aso, Satoka Browse this author |
Watanabe-Kusunoki, Kanako Browse this author |
Ueda, Yusho Browse this author |
Miyoshi, Atsuko Browse this author |
Hattanda, Fumihiko Browse this author |
Nishio, Saori Browse this author |
Uozumi, Ryo Browse this author |
Ishizu, Akihiro Browse this author |
Atsumi, Tatsuya Browse this author →KAKEN DB |
Keywords: | granulocytes |
low-density granulocytes |
neutrophil extracellular traps |
SARS-CoV-2 |
severe COVID-19 |
Issue Date: | Jan-2023 |
Publisher: | John Wiley & Sons |
Journal Title: | Febs open bio |
Volume: | 13 |
Issue: | 1 |
Start Page: | 60 |
End Page: | 71 |
Publisher DOI: | 10.1002/2211-5463.13500 |
Abstract: | Severe coronavirus disease 2019 (COVID-19) is characterized by acute respiratory distress syndrome and multiple organ dysfunction, in which the host immune response plays a pivotal role. Excessive neutrophil activation and subsequent superfluity of neutrophil extracellular traps (NETs) can lead to tissue damage, and several studies have shown the involvement of neutrophils in severe COVID-19. However, the detailed responses of each neutrophil subset to SARS-CoV-2 infection has not been fully described. To explore this issue, we incubated normal-density granulocytes (NDGs) and low-density granulocytes (LDGs) with different viral titers of SARS-CoV-2. NDGs form NETs with chromatin fibers in response to SARS-CoV-2, whereas LDGs incubated with SARS-CoV-2 display a distinct morphology with condensed nuclei and moderate transcriptional changes. Based on these transcriptional changes, we suggest that AGO2 possibly plays a role in LDG regulation in response to SARS-CoV-2. |
Type: | article |
URI: | http://hdl.handle.net/2115/88791 |
Appears in Collections: | 北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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