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Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model

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Title: Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model
Authors: Suzuki, Hisataka Browse this author
Ura, Katsuro Browse this author
Ukeba, Daisuke Browse this author
Suyama, Takashi Browse this author
Iwasaki, Norimasa Browse this author
Watanabe, Masatoki Browse this author
Matsuzaki, Yumi Browse this author
Yamada, Katsuhisa Browse this author
Sudo, Hideki Browse this author →KAKEN DB
Keywords: low back pain
intervertebral disc regeneration
ultra-purified clonogenic bone marrow-derived mesenchymal stem cell
ultra-purified alginate
Issue Date: Feb-2023
Publisher: MDPI
Journal Title: Cells
Volume: 12
Issue: 3
Start Page: 505
Publisher DOI: 10.3390/cells12030505
Abstract: Intervertebral disc (IVD) degeneration is a major cause of low back pain. However, treatments directly approaching the etiology of IVD degeneration and discogenic pain are not yet established. We previously demonstrated that intradiscal implantation of cell-free bioresorbable ultra-purified alginate (UPAL) gel promotes tissue repair and reduces discogenic pain, and a combination of ultra-purified, Good Manufacturing Practice (GMP)-compliant, human bone marrow mesenchymal stem cells (rapidly expanding clones; RECs), and the UPAL gel increasingly enhanced IVD regeneration in animal models. This study investigated the therapeutic efficacy of injecting a mixture of REC and UPAL non-gelling solution for discogenic pain and IVD regeneration in a rat caudal nucleus pulposus punch model. REC and UPAL mixture and UPAL alone suppressed not only the expression of TNF-alpha, IL-6, and TrkA (p < 0.01, respectively), but also IVD degeneration and nociceptive behavior compared to punching alone (p < 0.01, respectively). Furthermore, REC and UPAL mixture suppressed these expression levels and nociceptive behavior compared to UPAL alone (p < 0.01, respectively). These results suggest that this minimally invasive treatment strategy with a single injection may be applied to treat discogenic pain and as a regenerative therapy.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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