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Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model
| Title: | Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model |
| Authors: | Suzuki, Hisataka Browse this author | | Ura, Katsuro Browse this author | | Ukeba, Daisuke Browse this author | | Suyama, Takashi Browse this author | | Iwasaki, Norimasa Browse this author | | Watanabe, Masatoki Browse this author | | Matsuzaki, Yumi Browse this author | | Yamada, Katsuhisa Browse this author | | Sudo, Hideki Browse this author →KAKEN DB |
| Keywords: | low back pain | | intervertebral disc regeneration | | ultra-purified clonogenic bone marrow-derived mesenchymal stem cell | | ultra-purified alginate |
| Issue Date: | Feb-2023 |
| Publisher: | MDPI |
| Journal Title: | Cells |
| Volume: | 12 |
| Issue: | 3 |
| Start Page: | 505 |
| Publisher DOI: | 10.3390/cells12030505 |
| Abstract: | Intervertebral disc (IVD) degeneration is a major cause of low back pain. However, treatments directly approaching the etiology of IVD degeneration and discogenic pain are not yet established. We previously demonstrated that intradiscal implantation of cell-free bioresorbable ultra-purified alginate (UPAL) gel promotes tissue repair and reduces discogenic pain, and a combination of ultra-purified, Good Manufacturing Practice (GMP)-compliant, human bone marrow mesenchymal stem cells (rapidly expanding clones; RECs), and the UPAL gel increasingly enhanced IVD regeneration in animal models. This study investigated the therapeutic efficacy of injecting a mixture of REC and UPAL non-gelling solution for discogenic pain and IVD regeneration in a rat caudal nucleus pulposus punch model. REC and UPAL mixture and UPAL alone suppressed not only the expression of TNF-alpha, IL-6, and TrkA (p < 0.01, respectively), but also IVD degeneration and nociceptive behavior compared to punching alone (p < 0.01, respectively). Furthermore, REC and UPAL mixture suppressed these expression levels and nociceptive behavior compared to UPAL alone (p < 0.01, respectively). These results suggest that this minimally invasive treatment strategy with a single injection may be applied to treat discogenic pain and as a regenerative therapy. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/88934 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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