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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Moth sex chromatin probed by comparative genomic hybridization (CGH)
| Title: | Moth sex chromatin probed by comparative genomic hybridization (CGH) |
| Authors: | Sahara, Ken1 Browse this author →KAKEN DB | | Marec, František Browse this author | | Eickhoff, Ulrike Browse this author | | Traut, Walther Browse this author |
| Authors(alt): | 佐原, 健1 |
| Keywords: | Lepidoptera | | comparative genomic hybridization | | molecular evolution | | W chromosome |
| Issue Date: | Apr-2003 |
| Publisher: | National Research Council Canada |
| Journal Title: | Genome |
| Volume: | 46 |
| Issue: | 2 |
| Start Page: | 339 |
| End Page: | 342 |
| Abstract: | Comparative genomic hybridization (CGH) with a probe mixture of differently labeled genomic DNA from females and males highlighted the W chromosomes in mitotic plates and the W chromatin in polyploid interphase nuclei of the silkworm Bombyx mori, the flour moth Ephestia kuehniella, and the wax moth Galleria mellonella. The overproportionate fluorescence signal indicated an accumulation of repetitive sequences in the respective W chromosomes. Measurements of the fluorescence signals revealed two components, one that is present also in male DNA (non-W chromosomes) and another one that is present only in or preponderantly in female DNA (W chromosomes). While the W chromosomes of E. kuehniella and G. mellonella had both components, that of B. mori appeared to lack the latter component. Our results show that CGH can be applied to obtain a first estimate of the sequence composition of sex chromosomes in species from which otherwise little is known on the molecular level. |
| Description URI: | http://pubs.nrc-cnrc.gc.ca |
| Rights: | Copyright (c) 2003 National Research Council of Canada |
| Type: | article |
| URI: | http://hdl.handle.net/2115/891 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐原 健
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