HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >

Moth sex chromatin probed by comparative genomic hybridization (CGH)

Files in This Item:
Genome46(2).pdf1.16 MBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Moth sex chromatin probed by comparative genomic hybridization (CGH)
Authors: Sahara, Ken1 Browse this author →KAKEN DB
Marec, František Browse this author
Eickhoff, Ulrike Browse this author
Traut, Walther Browse this author
Authors(alt): 佐原, 健1
Keywords: Lepidoptera
comparative genomic hybridization
molecular evolution
W chromosome
Issue Date: Apr-2003
Publisher: National Research Council Canada
Journal Title: Genome
Volume: 46
Issue: 2
Start Page: 339
End Page: 342
Abstract: Comparative genomic hybridization (CGH) with a probe mixture of differently labeled genomic DNA from females and males highlighted the W chromosomes in mitotic plates and the W chromatin in polyploid interphase nuclei of the silkworm Bombyx mori, the flour moth Ephestia kuehniella, and the wax moth Galleria mellonella. The overproportionate fluorescence signal indicated an accumulation of repetitive sequences in the respective W chromosomes. Measurements of the fluorescence signals revealed two components, one that is present also in male DNA (non-W chromosomes) and another one that is present only in or preponderantly in female DNA (W chromosomes). While the W chromosomes of E. kuehniella and G. mellonella had both components, that of B. mori appeared to lack the latter component. Our results show that CGH can be applied to obtain a first estimate of the sequence composition of sex chromosomes in species from which otherwise little is known on the molecular level.
Description URI:
Rights: Copyright (c) 2003 National Research Council of Canada
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐原 健

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University