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Moth sex chromatin probed by comparative genomic hybridization (CGH)

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タイトル: Moth sex chromatin probed by comparative genomic hybridization (CGH)
著者: Sahara, Ken 著作を一覧する
Marec, František 著作を一覧する
Eickhoff, Ulrike 著作を一覧する
Traut, Walther 著作を一覧する
キーワード: Lepidoptera
comparative genomic hybridization
molecular evolution
W chromosome
発行日: 2003年 4月
出版者: National Research Council Canada
誌名: Genome
巻: 46
号: 2
開始ページ: 339
終了ページ: 342
抄録: Comparative genomic hybridization (CGH) with a probe mixture of differently labeled genomic DNA from females and males highlighted the W chromosomes in mitotic plates and the W chromatin in polyploid interphase nuclei of the silkworm Bombyx mori, the flour moth Ephestia kuehniella, and the wax moth Galleria mellonella. The overproportionate fluorescence signal indicated an accumulation of repetitive sequences in the respective W chromosomes. Measurements of the fluorescence signals revealed two components, one that is present also in male DNA (non-W chromosomes) and another one that is present only in or preponderantly in female DNA (W chromosomes). While the W chromosomes of E. kuehniella and G. mellonella had both components, that of B. mori appeared to lack the latter component. Our results show that CGH can be applied to obtain a first estimate of the sequence composition of sex chromosomes in species from which otherwise little is known on the molecular level.
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Rights: Copyright (c) 2003 National Research Council of Canada
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 佐原 健


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