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Hokkaido University Collection of Scholarly and Academic Papers >
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Enhancement of Vaccine-Induced T-Cell Responses by PD-L1 Blockade in Calves
| Title: | Enhancement of Vaccine-Induced T-Cell Responses by PD-L1 Blockade in Calves |
| Authors: | Okagawa, Tomohiro Browse this author →KAKEN DB | | Konnai, Satoru Browse this author →KAKEN DB | | Nakamura, Hayato Browse this author | | Ganbaatar, Otgontuya Browse this author | | Sajiki, Yamato Browse this author | | Watari, Kei Browse this author | | Noda, Haruka Browse this author | | Honma, Mitsuru Browse this author | | Kato, Yukinari Browse this author | | Suzuki, Yasuhiko Browse this author →KAKEN DB | | Maekawa, Naoya Browse this author →KAKEN DB | | Murata, Shiro Browse this author →KAKEN DB | | Ohashi, Kazuhiko Browse this author →KAKEN DB |
| Keywords: | PD-L1 | | PD-1 | | T cell | | live attenuated vaccine | | immunotherapy | | cattle |
| Issue Date: | 1-Mar-2023 |
| Publisher: | MDPI |
| Journal Title: | Vaccines |
| Volume: | 11 |
| Issue: | 3 |
| Start Page: | 559 |
| Publisher DOI: | 10.3390/vaccines11030559 |
| Abstract: | Interactions between programmed death 1 (PD-1) and PD-ligand 1 (PD-L1) cause functional exhaustion of T cells by inducing inhibitory signals, thereby attenuating effector functions of T cells. We have developed an anti-bovine PD-L1 blocking antibody (Ab) and have demonstrated that blockade of the interaction between PD-1 and PD-L1 reactivates T-cell responses in cattle. In the present study, we examined the potential utility of PD-1/PD-L1-targeted immunotherapy in enhancing T-cell responses to vaccination. Calves were inoculated with a hexavalent live-attenuated viral vaccine against bovine respiratory infections in combination with treatment with an anti-PD-L1 Ab. The expression kinetics of PD-1 in T cells and T-cell responses to viral antigens were measured before and after vaccination to evaluate the adjuvant effect of anti-PD-L1 Ab. PD-1 expression was upregulated in vaccinated calves after the administration of a booster vaccination. The activation status of CD4(+), CD8(+), and gamma delta TCR+ T cells was enhanced by the combination of vaccination and PD-L1 blockade. In addition, IFN-gamma responses to viral antigens were increased following combinatorial vaccination with PD-L1 blockade. In conclusion, the blockade of the PD-1/PD-L1 interaction enhances T-cell responses induced by vaccination in cattle, indicating the potential utility of anti-PD-L1 Ab in improving the efficacy of current vaccination programs. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/89155 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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