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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
The dynamics of the microbiome in Ixodidae are shaped by tick ontogeny and pathogens in Sarawak, Malaysian Borneo
| Title: | The dynamics of the microbiome in Ixodidae are shaped by tick ontogeny and pathogens in Sarawak, Malaysian Borneo |
| Authors: | Lau, Alice C. C. Browse this author | | Mohamed, Wessam Mohamed Ahmed Browse this author | | Nakao, Ryo Browse this author →KAKEN DB | | Onuma, Manabu Browse this author | | Qiu, Yongjin Browse this author | | Nakajima, Nobuyoshi Browse this author | | Shimozuru, Michito Browse this author →KAKEN DB | | Mohd-Azlan, Jayasilan Browse this author | | Moustafa, Mohamed Abdallah Mohamed Browse this author | | Tsubota, Toshio Browse this author →KAKEN DB |
| Keywords: | microbiome | | next-generation sequencing | | pathogens | | Sarawak | | ticks |
| Issue Date: | 9-Feb-2023 |
| Publisher: | Microbiology Society |
| Journal Title: | Microbial Genomics |
| Volume: | 9 |
| Issue: | 2 |
| Start Page: | 954 |
| Publisher DOI: | 10.1099/mgen.0.000954 |
| Abstract: | Tick-borne diseases have recently been considered a potential emerging public health threat in Malaysia; however, fundamental studies into tick-borne pathogens and microbiome appear limited. In this study, six tick species (Ixodes granulatus, Haemaphysa-lis hystricis, Haemaphysalis shimoga, Dermacentor compactus, Dermacentor steini and Dermacentor atrosignatus) collected from two primary forests and an oil palm plantation in Sarawak, Malaysian Borneo, were used for microbiome analysis targeting bacterial 16S rDNA using next-generation sequencing (NGS). In addition, bacterial species were further characterized in con-ventional PCRs to identify potential pathogens. Sequences generated from NGS were first filtered with the Decontam package in R before subsequent microbial diversity analyses. Alpha and beta analyses revealed that the genus Dermacentor had the highest microbial diversity, and H. shimoga significantly differed in microbial composition from other tick species. Alpha and beta diversities were also significantly different between developmental stages of H. shimoga. Furthermore, we observed that some bacterial groups were significantly more abundant in certain tick species and developmental stages of H. shimoga. We tested the relative abundances using pairwise linear discriminant analysis effect size (LEfSe), which also revealed significant microbial composition differences between Borrelia-positive and Borrelia-negative I. granulatus ticks. Finally, pathogenic and potentially pathogenic bacteria circulating in different tick species, such as Rickettsia heilongjiangensis, Ehrlichia sp., Anaplasma sp. and Bartonella spp. were characterized by PCR and sequencing. Moreover, Coxiella and Francisella- like potential symbionts were identified from H. shimoga and D. steini, respectively. More studies are required to unravel the factors associated with the variations observed in this study. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/89159 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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