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Expression dynamics of podoplanin in the mineralization of cultured osteoblasts with mechanostress
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| Title: | Expression dynamics of podoplanin in the mineralization of cultured osteoblasts with mechanostress |
| Other Titles: | 機械的ストレスを負荷した培養骨芽細胞の石灰化におけるポドプラニンの発現動態 |
| Authors: | 竹縄, 智紘 Browse this author |
| Keywords: | podoplanin | | mechanostress | | mineralization | | anti-podoplanin |
| Issue Date: | 22-Mar-2018 |
| Publisher: | Hokkaido University |
| Abstract: | Here, we report the expression dynamics of podoplanin in cultured bone marrow-derived osteoblasts with mechanical stress and the effect of anti-podolanin on the mineralization. The expression of podoplanin in cultured osteoblasts subjected to vertical elongation straining was significantly higher than that in cells without straining, and the expression increased with duration of elongation time as well as the expression for both osteopontin and osteocalcin tested as positive controls. The podoplanin mRNA amount in cultured osteoblasts subjected to straining in non-mineralization medium was significantly larger than in unstrained cells in mineralization medium. In the western blot analysis for the immunoprecipitated protein and in ELISA for the cell lysate, cultured osteoblasts increased podoplanin production with longer durations of elongation straining and reached a plateau within 3-5 days. There are significantly less of mineralization products in osteoblasts cultured with antibodies for podoplanin, as well as in cells with both antibodies for osteopontin, and osteocalcin as positive controls than in cells with medium only and with phenotype antibody controls. These findings may suggest that mechanostress induces the production of podoplanin, and that podoplanin may contribute to the mineralization in osteoblasts in bone under the circumstances with mechanostress. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第13041号 |
| Degree Level: | 博士 |
| Degree Discipline: | 歯学 |
| Examination Committee Members: | (主査) 特任教授 飯田 順一郎, 教授 網塚 憲生, 特任教授 鈴木 邦明 |
| Degree Affiliation: | 歯学研究科（口腔医学専攻） |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/89194 |
| Appears in Collections: | 課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)
学位論文 (Theses) > 博士 （歯学）
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