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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
In vitro cultivation of spermatocysts to matured sperm in the silkworm Bombyx mori
| Title: | In vitro cultivation of spermatocysts to matured sperm in the silkworm Bombyx mori |
| Authors: | Kawamura, Naoko Browse this author | | Sahara, Ken2 Browse this author →KAKEN DB |
| Authors(alt): | 佐原, 健2 |
| Keywords: | apyrene sperm | | Bombyx hemolymph | | eupyrene sperm | | in vitro cultivation | | silkworm |
| Issue Date: | 2002 |
| Publisher: | Blackwell Publishing, Inc. |
| Journal Title: | Development Growth & Differentiation |
| Volume: | 44 |
| Issue: | 4 |
| Start Page: | 273 |
| End Page: | 280 |
| Publisher DOI: | 10.1046/j.1440-169X.2002.00641.x |
| Abstract: | Bombyx spermatogonia are bipotential, producing nucleate eupyrene sperm and anucleate apyrene sperm. An in vitro cultivation of spermatocysts of Bombyx mori from spermatocytes to matured sperm was established. The present experiment made clear that: (i) spermatocysts must be isolated; (ii) constant shaking at 45 r.p.m. was necessary; and (iii) the addition of Bombyx hemolymph (BH) was indispensable for successful cultivation. In the absence of BH, spermatogenesis proceeded normally for 2 or 3 days and, thereafter, spermatocytes and sperm bundles began to degenerate. The best results for normal eupyrene spermatogenesis were obtained when culture medium containing BH of the corresponding stage was used in every exchange of the medium at 72 h intervals. None or only a small number of apyrene sperm bundles was produced by this culture system when spermatocysts from larval testes were used, although eupyrene spermatogenesis proceeded normally to form matured, or squeezed, sperm bundles. |
| Rights: | Copyright (c) 2002, Blackwell Publishing |
| Relation: | http://www.blackwellpublishing.com/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/892 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐原 健
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