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Upstream open reading frame-mediated upregulation of ANAC082 expression in response to nucleolar stress in Arabidopsis

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Title: Upstream open reading frame-mediated upregulation of ANAC082 expression in response to nucleolar stress in Arabidopsis
Authors: Sasaki, Shun Browse this author
Murakami, Toru Browse this author
Yasumuro, Miharu Browse this author
Makita, Ayaka Browse this author
Oi, Yutaro Browse this author
Hiragori, Yuta Browse this author
Watanabe, Shun Browse this author
Kudo, Rin Browse this author
Hayashi, Noriya Browse this author
Ohbayashi, Iwai Browse this author
Sugiyama, Munetaka Browse this author
Yamashita, Yui Browse this author →KAKEN DB
Naito, Satoshi Browse this author →KAKEN DB
Onouchi, Hitoshi Browse this author →KAKEN DB
Keywords: endoplasmic reticulum stress
nucleolar stress
ribosome biogenesis
upstream ORF
Issue Date: Mar-2023
Publisher: 日本植物バイオテクノロジー学会(Japanese Society for Plant Biotechnology)
Journal Title: Plant biotechnology
Volume: 40
Issue: 1
Start Page: 21
End Page: 30
Publisher DOI: 10.5511/plantbiotechnology.22.1215a
Abstract: Perturbations in ribosome biogenesis cause a type of cellular stress called nucleolar or ribosomal stress, which triggers adaptive responses in both animal and plant cells. The Arabidopsis ANAC082 transcription factor has been identified as a key mediator of the plant nucleolar stress response. The 5 '-untranslated region (5 '-UTR) of ANAC082 mRNA contains an upstream ORF (uORF) encoding an evolutionarily conserved amino acid sequence. Here, we report that this uORF mediates the upregulation of ANAC082 expression in response to nucleolar stress. When transgenic Arabidopsis plants containing a luciferase reporter gene under the control of the ANAC082 promoter and 5 '-UTR were treated with reagents that induced nucleolar stress, expression of the reporter gene was enhanced in a uORF sequence-dependent manner. Additionally, we examined the effect of an endoplasmic reticulum (ER) stress-inducing reagent on reporter gene expression because the closest homolog of ANAC082 in Arabidopsis, ANAC103, is involved in the ER stress response. However, the ANAC082 uORF did not respond to ER stress. Interestingly, although ANAC103 has a uORF with an amino acid sequence similar to that of the ANAC082 uORF, the C-terminal sequence critical for regulation is not well conserved among ANAC103 homologs in Brassicaceae. Transient expression assays revealed that unlike the ANAC082 uORF, the ANAC103 uORF does not exert a sequence-dependent repressive effect. Altogether, our findings suggest that the ANAC082 uORF is important for the nucleolar stress response but not for the ER stress response, and that for this reason, the uORF sequence-dependent regulation was lost in ANAC103 during evolution.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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