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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Water-soluble protein from walleye pollock (Gadus chalcogrammus) suppresses lipopolysaccharide-induced inflammation by attenuating TLR4-MyD88 expression in macrophages
| Title: | Water-soluble protein from walleye pollock (Gadus chalcogrammus) suppresses lipopolysaccharide-induced inflammation by attenuating TLR4-MyD88 expression in macrophages |
| Authors: | Ono, Masataka Browse this author | | Watari, Satomi Browse this author | | Nishizawa-Higashi, Mizuho Browse this author | | Konishi, Tatsuya Browse this author | | Takahashi, Yoshinori Browse this author | | Saeki, Hiroki Browse this author →KAKEN DB | | Joe, Ga-Hyun Browse this author →KAKEN DB |
| Keywords: | Fish water-soluble protein | | Macrophage | | Anti-inflammation | | LPS | | TLR4 | | MyD88 |
| Issue Date: | 2023 |
| Publisher: | Elsevier |
| Journal Title: | Food Chemistry: Molecular Sciences |
| Volume: | 6 |
| Start Page: | 100165 |
| Publisher DOI: | 10.1016/j.fochms.2023.100165 |
| Abstract: | Water-soluble protein (WSP) from fish meat is abundant in the waste effluent generated via the surimi manufacturing process. This study investigated the anti-inflammatory effects and mechanisms of fish WSP using primary macrophages (MO) and animal ingestion. MO were treated with digested-WSP (d-WSP, 500 mu g/mL) with or without lipopolysaccharide (LPS) stimulation. For the ingestion study, male ICR mice (5 weeks old) were fed 4% WSP for 14 days following LPS administration (4 mg/kg body weight). d-WSP decreased the expression of Tlr4, an LPS receptor. Additionally, d-WSP significantly suppressed the secretion of inflammatory cytokines, phagocytic ability, and Myd88 and Il1b expressions of LPS-stimulated macrophages. Furthermore, the ingestion of 4% WSP attenuated not only LPS-induced IL-10 secretion in the blood but also Myd88 and Il1b expressions in the liver. Thus, fish WSP decreases the expressions of the genes involved in the TLR4-MyD88 pathway in MO and the liver, thereby suppressing inflammation. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/89254 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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