|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Evaluating the sampling effort for the metabarcoding-based detection of fish environmental DNA in the open ocean
| Title: | Evaluating the sampling effort for the metabarcoding-based detection of fish environmental DNA in the open ocean |
| Authors: | Kawakami, Tatsuya Browse this author | | Yamazaki, Aya Browse this author | | Asami, Maki Browse this author | | Goto, Yuko Browse this author | | Yamanaka, Hiroki Browse this author | | Hyodo, Susumu Browse this author | | Ueno, Hiromichi Browse this author →KAKEN DB | | Kasai, Akihide Browse this author →KAKEN DB |
| Keywords: | asymptotic analysis | | Chukchi Sea | | dissimilarity | | northwestern Pacific Ocean | | replicated sampling |
| Issue Date: | Mar-2023 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Ecology and evolution |
| Volume: | 13 |
| Issue: | 3 |
| Start Page: | e9921 |
| Publisher DOI: | 10.1002/ece3.9921 |
| Abstract: | Clarifying the effect of the sampling protocol on the detection of environmental DNA (eDNA) is essential for appropriately designing biodiversity research. However, technical issues influencing eDNA detection in the open ocean, which consists of water masses with varying environmental conditions, have not been thoroughly investigated. This study evaluated the sampling effort for the metabarcoding-based detection of fish eDNA using replicate sampling with filters of different pore sizes (0.22 and 0.45 mu m) in the subtropical and subarctic northwestern Pacific Ocean and Arctic Chukchi Sea. The asymptotic analysis predicted that the accumulation curves for detected taxa did not saturate in most cases, indicating that our sampling effort (7 or 8 replicates, corresponding to 10.5-40 L of filtration in total) was insufficient to fully assess the species diversity in the open ocean and that tens of replicates or a substantial filtration volume were required. The Jaccard dissimilarities between filtration replicates were comparable with those between the filter types at any site. In subtropical and subarctic sites, turnover dominated the dissimilarity, suggesting that the filter pore size had a negligible effect. In contrast, nestedness dominated the dissimilarity in the Chukchi Sea, implying that the 0.22 mu m filter could collect a broader range of eDNA than the 0.45 mu m filter. Therefore, the effect of filter selection on the collection of fish eDNA likely varies depending on the region. These findings highlight the highly stochastic nature of fish eDNA collection in the open ocean and the difficulty of standardizing the sampling protocol across various water masses. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/89255 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|
