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Volume 39, Number 2-4 >

THE EFFECTIVENESS OF TRYPSIN TREATMENT TO REMOVE SENDAI VIRUS ADHERING TO THE ZONA PELLUCIDA OF MOUSE PREIMPLANTATION EMBRYOS

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.39.2-4.133

Title: THE EFFECTIVENESS OF TRYPSIN TREATMENT TO REMOVE SENDAI VIRUS ADHERING TO THE ZONA PELLUCIDA OF MOUSE PREIMPLANTATION EMBRYOS
Authors: LAVILLA-APELO, Carmencita Browse this author
KIDA, Hiroshi Browse this author →KAKEN DB
KANAGAWA, Hiroshi Browse this author
Keywords: mouse embryo
Sendai virus
washing
trypsin treatment
Issue Date: 26-Dec-1991
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 39
Issue: 2-4
Start Page: 133
End Page: 141
Abstract: The standard washing and trypsin treatment procedures to remove viruses adhering to the zona pellucida (ZP) were evaluated. Mouse embryos at the early blastocyst stage were exposed to Sendai virus, and then washed or treated with trypsin. Even after washing or trypsin treatment, Sendai virus was detected in the twelfth and final wash. The virus was still shown to adhere to the ZP by immunofluorescence assay. The embryos developed into expanded blastocysts following 24 hours of in vitro culture. Viral antigen was clearly demonstrated in the cells forming the expanded blastocysts, indicating that viral replication occurred in these cells. The present results suggest that the standard washing or trypsin treatment are not sufficient to remove Sendai virus adhering to the ZP of mouse embryos.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/8937
Appears in Collections:Japanese Journal of Veterinary Research > Volume 39, Number 2-4

Submitter: 獣医学部図書室

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