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Volume 39, Number 2-4 >

NUCLEOTIDE SEQUENCE OF A GENE ENCODING A NEW GENUS SPECIFIC PROTEIN OF CHLAMYDIA PSITTACI

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.39.2-4.159

Title: NUCLEOTIDE SEQUENCE OF A GENE ENCODING A NEW GENUS SPECIFIC PROTEIN OF CHLAMYDIA PSITTACI
Authors: SATO, Chiaki Browse this author
KATUMATA, Atushi Browse this author
TAKASHIMA, Ikuo Browse this author
HASHIMOTO, Nobuo Browse this author
Keywords: Chlamydia psittaci
Issue Date: 26-Dec-1991
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 39
Issue: 2-4
Start Page: 159
End Page: 165
Abstract: DNA fragment No. 13 from the C. psittaci pigeon strain, has been cloned in the plasmid pUC19. Hybridization analysis revealed that the fragment maintained a chlamydial common sequence. Furthermore, nucleotide seqencing identified two partial open reading frames (ORF), 675b. p. and 530b. p.. Expression of ORFs revealed that the second ORF encoded 25KD polypeptide, whereas the first ORF did not produce any antigenic product. The 25KD β-galactosidase fusion protein reacted strongly with chlamydia-specific antibodies elicited against a number of different chlamydial strains. Gene Bank analysis showed that this cloned gene is not highly homologous with chlamydia or other organisms for which nucleotide sequences have already been published. This 25KD polypeptide may be an additional genus-specific antigen of Chlamydiae.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/8939
Appears in Collections:Japanese Journal of Veterinary Research > Volume 39, Number 2-4

Submitter: 獣医学部図書室

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