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Development of new tools to study membrane-anchored mammalian Atg8 proteins

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Title: Development of new tools to study membrane-anchored mammalian Atg8 proteins
Authors: Park, Sang-Won Browse this author
Jeon, Pureum Browse this author
Yamasaki, Akinori Browse this author
Lee, Hye Eun Browse this author
Choi, Haneul Browse this author
Mun, Ji Young Browse this author
Jun, Yong-Woo Browse this author
Park, Ju-Hui Browse this author
Lee, Seung-Hwan Browse this author
Lee, Soo-Kyeong Browse this author
Lee, You-Kyung Browse this author
Song, Hyun Kyu Browse this author
Lazarou, Michael Browse this author
Cho, Dong-Hyong Browse this author
Komatsu, Masaaki Browse this author
Noda, Nobuo N. Browse this author
Jang, Deok-Jin Browse this author
Lee, Jin-A Browse this author
Keywords: Autophagy
LIR motif
mammalian ATG8
RavZ protein
selective mATG8-PE delipidation
Issue Date: 4-May-2023
Publisher: Taylor & Francis
Journal Title: Autophagy
Volume: 19
Issue: 5
Start Page: 1424
End Page: 1443
Publisher DOI: 10.1080/15548627.2022.2132040
Abstract: Mammals conserve multiple mammalian Atg8-family proteins (mATG8s) consisting of GABARAP (GABA type A receptor-associated protein) and MAP1LC3/LC3 (microtubule associated protein 1 light chain 3) subfamilies that tightly bind to autophagic membranes in a membrane-anchored form. These proteins are crucial for selective autophagy and recruit proteins bearing LC3-interacting region (LIR) motifs. However, because limited research tools are available, information on the specific roles of each membrane-anchored mATG8 in selective autophagy is scarce. In this study, we identified LIR motifs specific to the membrane-anchored form of each mATG8 and characterized the residues critical for their selective interaction using cell-based assays and structural analyses. We then used these selective LIR motifs to develop probes and irreversible deconjugases that targeted selective membrane-anchored mATG8s in the autophagic membrane, revealing that membrane-anchored GABARAP subfamily proteins regulate the aggrephagy of amyotrophic lateral sclerosis-linked protein aggregates. Our tools will be useful for elucidating the functional significance of each mATG8 protein on autophagic membranes in autophagy research.
Type: article
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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