| Title: | Direct detection of Mycobacterium bovis by a dry loop-mediated isothermal amplification assay in cattle samples collected during routine abattoir examination in Malawi |
| Authors: | Kapalamula, Thoko Flav Browse this author |
| Thapa, Jeewan Browse this author |
| Hayashida, Kyoko Browse this author |
| Chizimu, Joseph Browse this author |
| Tanomsridachchai, Wimonrat Browse this author |
| Nyenje, Mirriam Ethel Browse this author |
| Mkakosya, Rajab Browse this author |
| Nakajima, Chie Browse this author →KAKEN DB |
| Suzuki, Yasuhiko Browse this author →KAKEN DB |
| Keywords: | bovine tuberculosis |
| crude extracted DNA |
| dry LAMP assay |
| Mycobacterium bovis |
| Issue Date: | 8-Apr-2023 |
| Publisher: | SAGE Publications |
| Journal Title: | Journal of Veterinary Diagnostic Investigation |
| Volume: | 35 |
| Issue: | 3 |
| Start Page: | 307 |
| End Page: | 310 |
| Publisher DOI: | 10.1177/10406387231164596 |
| Abstract: | The lack of quick, accurate, and low-cost detection methods has hindered the active control strategies for bovine tuberculosis (bTB) in resource-limited countries with a high burden of disease. We developed a dry loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of Mycobacterium bovis, the principal causative agent of bTB, and evaluated the efficacy of the assay using suspected bTB samples collected during routine meat inspection at major regional abattoirs in Malawi. Template genomic DNA was extracted directly from the granulomatous bTB-like lesion (crude extracted DNA), as well as growth from the incubated mycobacterial growth indicator tubes (MGIT). Field results were visualized by the naked eye within 40 min following a color change of the amplified products. The sensitivity and specificity of the dry LAMP assay while using 152 DNA samples extracted from MGIT with confirmed M. bovis results were 98% and 88%, respectively. When 43 randomly selected crude DNA samples from lesions were used, the sensitivity and specificity of the dry LAMP assay were 100% and 75%, respectively. Our LAMP assay offers the potential to meet the demands for a low-cost and rapid field detection tool for bTB in resource-limited countries in which bTB is endemic. |
| Rights: | Kapalamula TF, Thapa J, Hayashida K, et al. Direct detection of Mycobacterium bovis by a dry loop-mediated isothermal amplification assay in cattle samples collected during routine abattoir examination in Malawi. Journal of Veterinary Diagnostic Investigation. 35(3) pp.307-310. Copyright © 2023 doi:10.1177/10406387231164596 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/89476 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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