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Light-sheet microscopy reveals dorsoventral asymmetric membrane dynamics of Amoeba proteus during pressure-driven locomotion
| Title: | Light-sheet microscopy reveals dorsoventral asymmetric membrane dynamics of Amoeba proteus during pressure-driven locomotion |
| Authors: | Taniguchi, Atsushi Browse this author | | Nishigami, Yukinori Browse this author →KAKEN DB | | Kajiura-Kobayashi, Hiroko Browse this author | | Takao, Daisuke Browse this author | | Tamaoki, Daisuke Browse this author | | Nakagaki, Toshiyuki Browse this author | | Nonaka, Shigenori Browse this author | | Sonobe, Seiji Browse this author |
| Keywords: | Amoeboid locomotion | | Cell locomotion | | Light sheet microscopy | | Membrane dynamics |
| Issue Date: | Feb-2023 |
| Publisher: | Company of Biologists |
| Journal Title: | Biology open |
| Volume: | 12 |
| Issue: | 2 |
| Start Page: | bio059671 |
| Publisher DOI: | 10.1242/bio.059671 |
| Abstract: | Amoebae are found all around the world and play an essential role in the carbon cycle in the environment. Therefore, the behavior of amoebae is a crucial factor when considering the global environment. Amoebae change their distribution through amoeboid locomotion, which are classified into several modes. In the pressure-driven mode, intracellular hydrostatic pressure generated by the contraction of cellular cortex actomyosin causes the pseudopod to extend. During amoeboid locomotion, the cellular surface exhibits dynamic deformation. Therefore, to understand the mechanism of amoeboid locomotion, it is important to characterize cellular membrane dynamics. Here, to clarify membrane dynamics during pressure-driven amoeboid locomotion, we developed a polkadot membrane staining method and performed light-sheet microscopy in Amoeba proteus, which exhibits typical pressure-driven amoeboid locomotion. It was observed that the whole cell membrane moved in the direction of movement, and the dorsal cell membrane in the posterior part of the cell moved more slowly than the other membrane. In addition, membrane complexity varied depending on the focused characteristic size of the membrane structure, and in general, the dorsal side was more complex than the ventral side. In summary, the membrane dynamics of Amoeba proteus during pressure-driven locomotion are asymmetric between the dorsal and ventral sides. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/89780 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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