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Serological and molecular epidemiological study on swine influenza in Zambia

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Title: Serological and molecular epidemiological study on swine influenza in Zambia
Authors: Harima, Hayato Browse this author →KAKEN DB
Okuya, Kosuke Browse this author →KAKEN DB
Kajihara, Masahiro Browse this author →KAKEN DB
Ogawa, Hirohito Browse this author →KAKEN DB
Simulundu, Edgar Browse this author
Bwalya, Eugene Browse this author
Qiu, Yongjin Browse this author →KAKEN DB
Mori-Kajihara, Akina Browse this author
Munyeme, Musso Browse this author
Sakoda, Yoshihiro Browse this author →KAKEN DB
Saito, Takehiko Browse this author →KAKEN DB
Hang'ombe, Bernard M. Browse this author
Sawa, Hirofumi Browse this author →KAKEN DB
Mweene, Aaron S. Browse this author
Takada, Ayato Browse this author →KAKEN DB
Keywords: influenza A virus
complete genome
Issue Date: 1-Nov-2021
Publisher: John Wiley & Sons
Journal Title: Transboundary and emerging diseases
Volume: 69
Issue: 4
Start Page: e931
End Page: e943
Publisher DOI: 10.1111/tbed.14373
Abstract: Influenza A viruses (IAVs) cause highly contagious respiratory diseases in humans and animals. In 2009, a swine-origin pandemic H1N1 IAV, designated A(H1N1)pdm09 virus, spread worldwide, and has since frequently been introduced into pig populations. Since novel reassortant IAVs with pandemic potential may emerge in pigs, surveillance for IAV in pigs is therefore necessary not only for the pig industry but also for public health. However, epidemiological information on IAV infection of pigs in Africa remains sparse. In this study, we collected 246 serum and 605 nasal swab samples from pigs in Zambia during the years 2011-2018. Serological analyses revealed that 49% and 32% of the sera collected in 2011 were positive for hemagglutination-inhibition (HI) and neutralizing antibodies against A(H1N1)pdm09 virus, respectively, whereas less than 5.3% of sera collected during the following period (2012-2018) were positive in both serological tests. The positive rate and the neutralization titres to A(H1N1)pdm09 virus were higher than those to classical swine H1N1 and H1N2 IAVs. On the other hand, the positive rate for swine H3N2 IAV was very low in the pig population in Zambia in 2011-2018 (5.3% and 0% in HI and neutralization tests, respectively). From nasal swab samples, we isolated one H3N2 and eight H1N1 IAV strains with an isolation rate of 1.5%. Phylogenetic analyses of all eight gene segments revealed that the isolated IAVs were closely related to human IAV strains belonging to A(H1N1)pdm09 and seasonal H3N2 lineages. Our findings indicate that reverse zoonotic transmission from humans to pigs occurred during the study period in Zambia and highlight the need for continued surveillance to monitor the status of IAVs circulating in swine populations in Africa.
Rights: This is the peer reviewed version of the following article: [Harima, H., Okuya, K., Kajihara, M., Ogawa, H., Simulundu, E., Bwalya, E., Qiu, Y., Mori-Kajihara, A., Munyeme, M., Sakoda, Y., Saito, T., Hang'ombe, B. M., Sawa, H., Mweene, A. S., & Takada, A. (2022). Serological and molecular epidemiological study on swine influenza in Zambia. Transboundary and Emerging Diseases, 69, e931– e943.]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Type: article (author version)
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高田 礼人

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