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Simultaneous quantification of imidacloprid and its metabolites in tissues of mice upon chronic low-dose administration of imidacloprid

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Title: Simultaneous quantification of imidacloprid and its metabolites in tissues of mice upon chronic low-dose administration of imidacloprid
Authors: Nimako, Collins Browse this author
Ikenaka, Yoshinori Browse this author →KAKEN DB
Akoto, Osei Browse this author
Fujioka, Kazutoshi Browse this author
Taira, Kumiko Browse this author →KAKEN DB
Arizono, Koji Browse this author →KAKEN DB
Kato, Keisuke Browse this author →KAKEN DB
Takahashi, Keisuke Browse this author
Nakayama, Shouta M. M. Browse this author →KAKEN DB
Ichise, Takahiro Browse this author
Ishizuka, Mayumi Browse this author →KAKEN DB
Keywords: Imidacloprid
Issue Date: 30-Aug-2021
Publisher: Elsevier
Journal Title: Journal of Chromatography A
Volume: 1652
Start Page: 462350
Publisher DOI: 10.1016/j.chroma.2021.462350
Abstract: This study aimed to (i) develop a sensitive method for simultaneous detection and quantification of imidacloprid (IMI) and seven of its metabolites in tissue specimens, and to (ii) determine the biodistribution of the IMI compounds in tissues of C57BL/6J male mice; after exposure to 0.6 mg/kg bw/day of IMI (10% of no observable adverse effect level of IMI) through a powdered diet for 24 weeks. We successfully developed a method which was accurate (recoveries were >= 70% for most compounds), sensitive (LODs <= 0.47 ng/mL and LOQs <= 1.43 ng/mL were recorded for all detected compounds, R-2 >= 0.99) and precise (RSDs <= 20%) for routine analysis of IMI and seven of its metabolites in blood and various tissue matrices. After bio-distributional analysis, IMI and five of its metabolites were detected in mice. Brain, testis, lung, kidney, inguinal white adipose tissue and gonadal white adipose tissue mainly accumulated IMI, blood and mesenteric white adipose tissue mainly accumulated IMI-olefin; liver mainly accumulated desnitroIMI; pancreas predominately accumulated 4-hydroxy-IMI. The desnitro-dehydro-IMI and the desnitro-IMI metabolites recorded tissue-blood concentration ratios >= 1.0 for testis, brain, lung and kidney. The cumulative levels of the six detected IMI compounds (Sigma 6 IMI compounds) were found in the decreasing order: blood > testis > brain > kidney > lung > iWAT > gWAT > mWAT > liver > pancreas. Altogether, this study provided essential data needed for effective mechanistic elucidation of compound-specific adverse outcomes associated with chronic exposures to IMI in mammalian species. (C) 2021 Elsevier B.V. All rights reserved.
Rights: © <2021>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 池中 良徳

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