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Mycosporine-like Amino Acids from Red Alga Dulse (Devaleraea inkyuleei) : Monthly Variation and Improvement in Extraction
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| Title: | Mycosporine-like Amino Acids from Red Alga Dulse (Devaleraea inkyuleei) : Monthly Variation and Improvement in Extraction |
| Authors: | Yamamoto, Ryuya Browse this author | | Takizawa, Koki Browse this author | | Miyabe, Yoshikatsu Browse this author | | Mune Mune, Martin Alain Browse this author | | Kishimura, Hideki Browse this author →KAKEN DB | | Kumagai, Yuya Browse this author →KAKEN DB |
| Keywords: | red alga | | dulse | | mycosporine-like amino acids | | monthly variation | | water-soluble protein | | phycoerythrin | | floridean starch | | xylan | | Usujiri Hokkaido |
| Issue Date: | 7-Sep-2023 |
| Publisher: | MDPI |
| Journal Title: | Phycology |
| Volume: | 3 |
| Issue: | 3 |
| Start Page: | 394 |
| End Page: | 404 |
| Publisher DOI: | 10.3390/phycology3030026 |
| Abstract: | Mycosporine-like amino acids (MAAs) are natural UV-absorbing compounds found inmicroalgae and macroalgae. The content of MAAs in algae varies with the seasons and environ-mental factors. Red alga dulse in Usujiri (Hokkaido, Japan) is an underutilized resource. Therefore,we investigated the amount of MAAs in Usujiri dulse in 2022 to clarify the suitable months forMAA extraction. In addition, we also evaluated the extraction method focusing on the extractionvolume. MAAs were prepared via the 20 volumes of 25% ethanol extraction method and detectedvia HPLC. The results showed that the amount of MAAs on 25 March 2022 showed the highestvalue ( 40.4 μmol/g DW) among the samples from 24 January to 13 May. The tendency of suitablesamples for MAA preparation corresponded to the term from mid-February to early April, whichwas the same as the previous three years. Although the surveys from 2019-2021 were performed byusing the successive water-methanol method, it was found that the improved method also reflectedthe monthly variation in MAAs. The extraction of MAAs was performed via 20 or 40 volumes of 25% ethanol at 4 ℃ for 24 h. The amount of MAAs with 40 volumes of 25% ethanol extractionincreased 1.3-fold compared to that with 20 volumes of 25% ethanol extraction. These data are usefulinformation for valuable compound extraction from Usujiri dulse. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/90365 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山本 竜矢
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