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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
Peer-reviewed Journal Articles, etc >
Effects of Interfacial Interactions on Electrocatalytic Activity of Cytochrome c Oxidase in Biomimetic Lipid Membranes on Gold Electrodes
| Title: | Effects of Interfacial Interactions on Electrocatalytic Activity of Cytochrome c Oxidase in Biomimetic Lipid Membranes on Gold Electrodes |
| Authors: | Kato, Masaru Browse this author →KAKEN DB | | Sano, Ryoya Browse this author | | Yoshida, Narumi Browse this author | | Iwafuji, Masatoshi Browse this author | | Nishiyama, Yoshito Browse this author | | Oka, Sayuki Browse this author | | Shinzawa-Itoh, Kyoko Browse this author →KAKEN DB | | Nishida, Yuya Browse this author →KAKEN DB | | Shintani, Yasunori Browse this author →KAKEN DB | | Yagi, Ichizo Browse this author →KAKEN DB |
| Issue Date: | 6-Oct-2022 |
| Publisher: | American Chemical Society(ACS) |
| Journal Title: | The Journal of Physical Chemistry Letters |
| Volume: | 13 |
| Issue: | 39 |
| Start Page: | 9165 |
| End Page: | 9170 |
| Publisher DOI: | 10.1021/acs.jpclett.2c01765 |
| Abstract: | Effects of interfacial interactions on the electrocatalytic activity of protein-tethered bilayer lipid membranes (ptBLMs) containing cytochrome c oxidase (CcO) for the oxygen reduction reaction are studied by using protein film electrochemistry and surface-enhanced infrared absorption (SEIRA) spectroscopy. Mammalian CcO was immobilized on a gold electrode via self-assembled monolayers (SAMs) of mixed alkanethiols. The protein orientation on the electrode is controlled by SAM–CcO interactions and is critical to the cytochrome c (cyt c) binding. The CcO–phospholipid and CcO–cyt c interactions modulate the electrocatalytic activity of CcO, and more densely packed ptBLMs show higher electrocatalytic activity. Our study indicates that spectroscopic and electrochemical studies of ptBLMs can provide insights into the effects of relatively weak protein–protein and protein–lipid interactions on the enzymatic activity of transmembrane enzymes. |
| Rights: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in
Journal of Physical Chemistry Letters, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/articlesonrequest/AOR-KN3PSZBSXRVWBQ7EDI2J. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/90417 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 加藤 優
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