| Title: | Immunolocalization of endomucin-reactive blood vessels and a-smooth muscle actin-positive cells in murine nasal conchae |
| Authors: | Maruoka, Haruhi Browse this author →KAKEN DB |
| Hasegawa, Tomoka Browse this author →KAKEN DB |
| Yoshino, Hirona Browse this author |
| Abe, Miki Browse this author |
| Haraguchi-Kitakamae, Mai Browse this author |
| Yamamoto, Tomomaya Browse this author →KAKEN DB |
| Hongo, Hiromi Browse this author →KAKEN DB |
| Nakanishi, Ko Browse this author →KAKEN DB |
| Nasoori, Alireza Browse this author →KAKEN DB |
| Nakajima, Yuhi Browse this author |
| Omaki, Masayuki Browse this author |
| Sato, Yoshiaki Browse this author →KAKEN DB |
| Luiz de Freitas, Paulo Henrique Browse this author |
| Li, Minqi Browse this author →KAKEN DB |
| Keywords: | Nasal bone |
| Blood vessel |
| Endomucin |
| Alpha smooth muscle actin |
| Issue Date: | 19-Sep-2022 |
| Publisher: | Elsevier |
| Journal Title: | Journal of oral biosciences |
| Volume: | 64 |
| Issue: | 3 |
| Start Page: | 337 |
| End Page: | 345 |
| Publisher DOI: | 10.1016/j.job.2022.05.001 |
| PMID: | 35589073 |
| Abstract: | Objectives: Recently, the biological functions of endomucin-positive blood vessels and closely associated aSMA-positive cells in long bones have been highlighted. The surrounding tissues of the flat bones, such as nasal bones covered with mucosa and lamina propria, are different from those of the long bones, indicating the different distributions of endomucin-positive blood vessels and aSMA-reactive cells in nasal bones. This study demonstrates the immunolocalization of endomucin-reactive blood vessels and aSMA-positive cells in the nasal conchae of 3- and 7-week-old mice. Methods: The nasal conchae of 3-week-old and 7-week-old male C57BL/6J mice were used for immunoreaction of endomucin, CD34, PDGFbb, TRAP, and c-kit. Results: While we identified abundant endomucin-reactive blood vessels in the lamina propria neighboring the bone, not all were positive for endomucin. More CD34-reactive cells and small blood vessels were observed in the nasal conchae of 3-week-old mice than in those of 7-week-old mice. Some aSMApositive cells in the nasal conchae surrounded the blood vessels, indicating vascular smooth muscle cells, while other aSMA-immunopositive fibroblastic cells were detected throughout the lamina propria. aSMA-positive cells did not co-localize with c-kit-immunoreactivity, thereby indicating that the aSMApositive cells may be myofibroblasts rather than undifferentiated mesenchymal cells. Conclusions: Unlike long bones, nasal conchae contain endomucin-positive as well as endomucinnegative blood vessels and exhibit numerous aSMA-positive fibroblastic cells throughout the lamina propria neighboring the bone. Apparently, the distribution patterns of endomucin-positive blood vessels and aSMA-positive cells in nasal conchae are different from those in long bones. (c) 2022 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved. |
| Rights: | © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/90420 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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