| Title: | Histochemical examination of blood vessels in murine femora with intermittent PTH administration |
| Authors: | Maruoka, Haruhi Browse this author →KAKEN DB |
| Zhao, Shen Browse this author |
| Yoshino, Hirona Browse this author |
| Abe, Miki Browse this author |
| Yamamoto, Tomomaya Browse this author →KAKEN DB |
| Hongo, Hiromi Browse this author →KAKEN DB |
| Haraguchi-Kitakamae, Mai Browse this author |
| Nasoori, Alireza Browse this author →KAKEN DB |
| Ishizu, Hotaka Browse this author |
| Nakajima, Yuhi Browse this author |
| Omaki, Masayuki Browse this author |
| Shimizu, Tomohiro Browse this author →KAKEN DB |
| Iwasaki, Norimasa Browse this author →KAKEN DB |
| de Freitas, Paulo Henrique Luiz Browse this author |
| Li, Minqi Browse this author →KAKEN DB |
| Hasegawa, Tomoka Browse this author →KAKEN DB |
| Keywords: | Blood vessels |
| Perivascular stromal cells |
| Parathyroid hormone (PTH) |
| Endomucin |
| a SMA |
| Issue Date: | 19-Sep-2022 |
| Publisher: | Elsevier |
| Journal Title: | Journal of oral biosciences |
| Volume: | 64 |
| Issue: | 3 |
| Start Page: | 329 |
| End Page: | 336 |
| Publisher DOI: | 10.1016/j.job.2022.05.003 |
| PMID: | 35584768 |
| Abstract: | Objective: To verify the biological effects of parathyroid hormone (PTH) on the blood vessels in the bone, this study aimed to investigate histological alterations in endomucin-positive blood vessels and perivascular cells in murine femora after intermittent PTH administration. For comparison with blood vessels in the bone, we examined the distribution of endomucin-positive blood vessels and surrounding aSMAimmunoreactive perivascular cells in the liver, kidney, and aorta with or without PTH administration. Methods: Six-week-old male C57BL/6J mice received hPTH [1-34] or vehicle for two weeks. All mice were fixed with a paraformaldehyde solution after euthanasia, and the right femora, kidney, liver, and aorta were extracted for immunohistochemical analysis of endomucin, aSMA, ephrinB2, EphB4, and HIF1a. Light microscopic observations of semi-thin sections and transmission electron microscopic (TEM) observations of ultra-thin sections were performed on the left femora. Results: After intermittent PTH administration, aSMA-reactive/ephrinB2-positive stromal cells appeared around endomucin-positive/EphB4-immunoreactive blood vessels in the bone. In addition, intense immunoreactivities of EphB4 and HIF1a were seen in vascular endothelial cells after the PTH treatment. Several stromal cells surrounding PTH-treated blood vessels exhibited well-developed rough endoplasmic reticulum under TEM observations. In contrast to bone tissues, aSMA-positive stromal cells did not increase around the endomucin-positive blood vessels in the kidney, liver, or aorta, even after PTH administration. Conclusion: These findings show that intermittent PTH administration increases aSMA-reactive/ephrinB2-positive perivascular stromal cells in bone tissue but not in the kidney, liver, or aorta, suggesting that PTH preferentially affects blood vessels in the bone. (c) 2022 Published by Elsevier B.V. on behalf of Japanese Association for Oral Biology. |
| Rights: | © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/90422 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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