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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
Peer-reviewed Journal Articles, etc >
The canonical smooth muscle cell marker TAGLN is present in endothelial cells and is involved in angiogenesis
| Title: | The canonical smooth muscle cell marker TAGLN is present in endothelial cells and is involved in angiogenesis |
| Authors: | Tsuji-Tamura, Kiyomi Browse this author →KAKEN DB | | Morino-Koga, Saori Browse this author | | Suzuki, Shingo Browse this author →KAKEN DB | | Ogawa, Minetaro Browse this author →KAKEN DB |
| Keywords: | TAGLN | | Angiogenesis | | Endothelial cells | | Cell elongation |
| Issue Date: | 2-Aug-2021 |
| Publisher: | The Company of Biologists |
| Journal Title: | Journal of Cell Science |
| Volume: | 134 |
| Issue: | 15 |
| Start Page: | jcs254920 |
| Publisher DOI: | 10.1242/jcs.254920 |
| PMID: | 34338296 |
| CiNii Research CRID: | 1360013168766241536 |
| Abstract: | Elongation of vascular endothelial cells (ECs) is an important process in angiogenesis; however, the molecular mechanisms remain unknown. The actin-crosslinking protein TAGLN (transgelin, also known as SM22 or SM22α) is abundantly expressed in smooth muscle cells (SMCs) and is widely used as a canonical marker for this cell type. In the course of studies using mouse embryonic stem cells (ESCs) carrying an Tagln promoter-driven fluorescence marker, we noticed activation of the Tagln promoter during EC elongation. Tagln promoter activation co-occurred with EC elongation in response to vascular endothelial growth factor A (VEGF-A). Inhibition of phosphoinositide 3-kinase (PI3K)–Akt signaling and mTORC1 also induced EC elongation and Tagln promoter activation. Human umbilical vein endothelial cells (HUVECs) elongated, activated the TAGLN promoter and increased TAGLN transcripts in an angiogenesis model. Genetic disruption of TAGLN augmented angiogenic behaviors of HUVECs, as did the disruption of TAGLN2 and TAGLN3 genes. Tagln expression was found in ECs in mouse embryos. Our results identify TAGLN as a putative regulator of angiogenesis whose expression is activated in elongating ECs. This finding provides insight into the cytoskeletal regulation of EC elongation and an improved understanding of the molecular mechanisms underlying the regulation of angiogenesis. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/90462 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田村（辻） 潔美
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