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Biological behavior of myoepithelial cells in the regeneration of rat atrophied sublingual glands following release from duct ligation

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Title: Biological behavior of myoepithelial cells in the regeneration of rat atrophied sublingual glands following release from duct ligation
Authors: Takahashi, S.1 Browse this author →KAKEN DB
Kohgo, T. Browse this author
Nakamura, S. Browse this author
Arambawatta, A. K. S. Browse this author
Domon, T. Browse this author →KAKEN DB
Wakita, M. Browse this author
Authors(alt): 高橋, 茂1
Keywords: parotid-gland
nuclear antigen
Issue Date: 9-Nov-2005
Publisher: Springer Science+Business Media B.V., Formerly Kluwer Academic Publishers B.V.
Journal Title: Journal of Molecular Histology
Volume: 36
Issue: 5
Publisher DOI: 10.1007/s10750-005-0883-0
Abstract: The present study aimed to clarify how myoepithelial cells behave during regeneration of an atrophied sublingual gland by investigating cell proliferation and ultrastructure. Atrophy of rat sublingual glands was induced by unilateral ligation of the excretory duct near the hilum with metal clips, which were then removed after one week of ligation for regeneration. The sublingual glands 0–14 days after unligation were examined with single immunohistochemistry for actin as a marker of myoepithelial cells, double immunohistochemistry for actin and proliferating cell nuclear antigen (PCNA) as a marker of proliferating cells, and transmission electron microscopy (TEM). The single immunohistochemistry and TEM showed that myoepithelial cells surrounded residual ducts in the atrophied glands and immature and mature acini in the regenerating glands. Although PCNA-positive myoepithelial cells were identified during regeneration, PCNA labeling indices of myoepithelial cells were low at all time points except at day 7. Ultrastructurally, myoepithelial cells showing bizarre shaped structures in the atrophy changed with maturation of differentiating acinar cells and appeared normal in the regenerated glands. There was no differentiation of the remaining duct cells to myoepithelial cells. These observations suggest that proliferation of myoepithelial cells and differentiation to myoepithelial cells do not commonly participate in the regeneration of atrophied sublingual glands and that the bizarre shaped myoepithelial cells in the atrophied sublingual glands recover the original shapes with acinar cell regeneration.
Rights: The original publication is available at
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 茂

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