19F-NMR測定によるリポソームを生体膜モデルとしたイソフルランの作用部位の研究

本間, 将一; 平沖, 敏文; 渋谷, 真希子; 鈴木, 邦明; 藤澤, 俊明


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Hokkaido University Collection of Scholarly and Academic Papers >
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北海道歯学雑誌 >
第44巻 >

19F-NMR測定によるリポソームを生体膜モデルとしたイソフルランの作用部位の研究

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Title:	19F-NMR測定によるリポソームを生体膜モデルとしたイソフルランの作用部位の研究
Other Titles:	Effect of isoflurane on multilamellar liposome-Analysis by 19F-NMR
Authors:	本間, 将一¹ Browse this author
	平沖, 敏文² Browse this author →KAKEN DB
	渋谷, 真希子³ Browse this author →KAKEN DB
	鈴木, 邦明⁴ Browse this author →KAKEN DB
	藤澤, 俊明⁵ Browse this author →KAKEN DB
Authors(alt):	Honma, Shoichi¹
	Hiraoki, Toshifumi²
	Shibuya, Makiko³
	Suzuki, Kuniaki⁴
	Fujisawa, Toshiaki⁵
Keywords:	19F-NMR
	isoflurane
	liposome
	the action site
Issue Date:	15-Sep-2023
Publisher:	北海道歯学会
Journal Title:	北海道歯学雑誌
Volume:	44
Start Page:	49
End Page:	60
Abstract:	[Objectives] To investigate the interaction of a general anesthetic with biological membranes, we measured 19F nuclear magnetic resonance (NMR) spectra of isoflurane on the isoflurane-multilamellar liposome (MLV) system. The paramagnetic effect of the spin-probe was examined to obtain the spatial structure between isoflurane and spin-probe in the MLV. [Methods] We made the MLV solution including spin probe, 5- or 16-doxyl stearic acid (5- or16-DSA), added isoflurane to the MLV solution and obtained chemical shifts, the spin-lattice relaxation time (T1) and the spin-spin relaxation time (T2) of isoflurane on the spectra at 7T. [Results and Discussion] The addition of isoflurane did not change the chemical shifts and the T1, but line widths became slightly broad and the T2 decreased remarkably, suggesting that isoflurane affects a chemical-exchange process between free and bound isoflurane on MLV. T1 and the T2 furthermore decreased in the presence of 5-DSA or 16-DSA due to the paramagnetic interaction between 19F nuclei of isoflurane and the electron of the spin-probe. T1 and T2 with 5-DSA were shorter than those of 16-DSA. These results suggest that the di stance between isoflurane and 5-DSA in MLV is closer than that of 16-DSA, and that isoflurane molecules locate in the outer surface of MLV.
Type:	article
URI:	http://hdl.handle.net/2115/90506
Appears in Collections:	北海道歯学雑誌 > 第44巻

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