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Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions

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Title: Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions
Authors: Fukura, Koki Browse this author
Tanaka, Ayumi Browse this author
Tanaka, Ryouichi Browse this author
Ito, Hisashi Browse this author
Keywords: Arabidopsis
Chlorophyll breakdown
Chlorophyll b reductase
Thylakoid fractionation
Grana margin
Issue Date: 1-Nov-2021
Publisher: Elsevier
Journal Title: Journal of plant physiology
Volume: 266
Start Page: 153535
Publisher DOI: 10.1016/j.jplph.2021.153535
Abstract: During leaf senescence, chlorophyll a and b are degraded through several enzymatic reactions, including chlorophyll b reductase, 7-hydroxymethyl chlorophyll a reductase, and Mg-dechelatase. Considering that the intermediates of the chlorophyll breakdown pathway are highly photoreactive, cooperative and efficient reactions of chlorophyll metabolic enzymes may protect chloroplasts from potential photo-oxidative damage. Here, we investigated the sub-organellar localization and cooperative reactions of the enzymes involved in the chlorophyll breakdown pathway by the fractionation of thylakoid membranes and enzymatic assays using recombinant proteins. We found that these enzymes were enriched in the grana margin fraction. Furthermore, we found that chlorophyll b reductase and Mg-dechelatase efficiently catabolized chlorophylls bound to the chlorophyll-protein complexes when these two enzymes were mixed. These results suggest that the co-localization of chlorophyll catabolic enzymes enables efficient chlorophyll breakdown. The results from this study highlight a key step forward in the investigation of the photosystem breakdown process.
Rights: ©2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 伊藤 寿

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