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Hokkaido University Collection of Scholarly and Academic Papers >
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Sulfotransferases (SULTs), enzymatic and genetic variation in Carnivora : Limited sulfation capacity in pinnipeds
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| Title: | Sulfotransferases (SULTs), enzymatic and genetic variation in Carnivora : Limited sulfation capacity in pinnipeds |
| Authors: | Kondo, Mitsuki Browse this author | | Ikenaka, Yoshinori Browse this author | | Nakayama, Shouta M. M. Browse this author | | Kawai, Yusuke K. Browse this author | | Mizukawa, Hazuki Browse this author | | Mitani, Yoko Browse this author | | Nomyama, Kei Browse this author | | Tanabe, Shinsuke Browse this author | | Ishizuka, Mayumi Browse this author →KAKEN DB |
| Keywords: | Wildlife | | Xenobiotic metabolism | | In silico analysis | | Genome database | | Phase II metabolism |
| Issue Date: | Jan-2023 |
| Publisher: | Elsevier |
| Journal Title: | Comparative biochemistry and physiology Part C: Toxicology & pharmacology |
| Volume: | 263 |
| Start Page: | 109476 |
| Publisher DOI: | 10.1016/j.cbpc.2022.109476 |
| Abstract: | Wild carnivorans are one of the most important species due to their high positions in the food chain. They are also highly affected by numerous environmental contaminants through bioaccumulation and biomagnification. Xenobiotic metabolism is a significant chemical defense system from xenobiotics because it degrades the activity of a wide range of chemicals, generally into less active forms, resulting in their deactivation. Sulfotransferases (SULTs) are one of the most important xenobiotic metabolic enzymes, which catalyze the sulfonation of a variety of endogenous and exogenous chemicals, such as hormones, neurotransmitters, and a wide range of xenobiotic compounds. Although SULTs are of such high importance, little research has focused on these enzymes in wild carnivorans. In this study, we clarified the genetic properties of SULTs in a wide range of mammals, focusing on carnivorans, using in silico genetic analyses. We found genetic deficiencies of SULT1E1 and SULT1D1 isoforms in all pinnipeds analyzed and nonsense mutations in SULT1Cs in several carnivorans including pinnipeds. We further investigated the enzymatic activity of SULT1E1 in vitro using liver cytosols from pinnipeds. Using a SULT1E1 probe substrate, we found highly limited estradiol sulfonation in pinnipeds, whereas other mammals had relatively high sulfation. These results suggest that pinnipeds have severely or completely absent SULT1E1 activity, which importantly catalyzes the metabolism of estrogens, drugs, and environmental toxins. This further implies a high susceptibility to a wide range of xenobiotics in these carnivorans, which are constantly exposed to environmental chemicals throughout their lifetime. |
| Rights: | © 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/91043 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 石塚 真由美
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