|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Effects of hemicelluloses on dehydrogenative polymerization of monolignols with cationic cell wall-bound peroxidase
This item is licensed under:Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
| Title: | Effects of hemicelluloses on dehydrogenative polymerization of monolignols with cationic cell wall-bound peroxidase |
| Authors: | Lyu, Yan Browse this author | | Suzuki, Shiori Browse this author →KAKEN DB | | Nagano, Hiroki Browse this author | | Shigetomi, Kengo Browse this author →KAKEN DB | | Tamai, Yutaka Browse this author →KAKEN DB | | Tsutsumi, Yuji Browse this author | | Uraki, Yasumitsu Browse this author →KAKEN DB |
| Keywords: | Dehydrogenation polymer | | Sinapyl alcohol | | Cationic cell wall-bound peroxidase | | Hemicellulose | | Partially acetylated xylan |
| Issue Date: | 1-Feb-2023 |
| Publisher: | Elsevier |
| Journal Title: | Carbohydrate Polymers |
| Volume: | 301 |
| Start Page: | 120305 |
| Publisher DOI: | 10.1016/j.carbpol.2022.120305 |
| Abstract: | To elucidate the influence of polysaccharides on hardwood lignification, dehydrogenative polymerization of monolignols, coniferyl alcohol (CA) and sinapyl alcohol (SA), was attempted with recombinant cationic cell wall-bound peroxidase (rCWPO-C) and horseradish peroxidase (HRP) in measurement cells of a quartz crystal mi-crobalance with dissipation (QCM-D). Hardwood cellulose nanofibers were anchored; hemicelluloses, xylan, partially acetylated xylan (AcXY), galactoglucomannan, and xyloglucan, and the enzymes were subsequently adsorbed onto the QCM-D sensor surface, enabling fabrication of artificial polysaccharide matrices. The largest amount of rCWPO-C is found to be adsorbed onto AcXY among all the polysaccharides, which affords the largest amount and size of spherical dehydrogenation polymers (DHPs) from both CA and SA. In contrast, no DHP and a small amount of DHPs are formed from SA and CA, respectively, by HRP catalysis in all of the polysaccharide matrices. This study demonstrates important functions of a real tree-derived peroxidase, rCWPO-C, and AcXY for hardwood lignification. |
| Rights: | © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/91104 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 鈴木 栞
|
