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Effects of hemicelluloses on dehydrogenative polymerization of monolignols with cationic cell wall-bound peroxidase

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Title: Effects of hemicelluloses on dehydrogenative polymerization of monolignols with cationic cell wall-bound peroxidase
Authors: Lyu, Yan Browse this author
Suzuki, Shiori Browse this author →KAKEN DB
Nagano, Hiroki Browse this author
Shigetomi, Kengo Browse this author →KAKEN DB
Tamai, Yutaka Browse this author →KAKEN DB
Tsutsumi, Yuji Browse this author
Uraki, Yasumitsu Browse this author →KAKEN DB
Keywords: Dehydrogenation polymer
Sinapyl alcohol
Cationic cell wall-bound peroxidase
Partially acetylated xylan
Issue Date: 1-Feb-2023
Publisher: Elsevier
Journal Title: Carbohydrate Polymers
Volume: 301
Start Page: 120305
Publisher DOI: 10.1016/j.carbpol.2022.120305
Abstract: To elucidate the influence of polysaccharides on hardwood lignification, dehydrogenative polymerization of monolignols, coniferyl alcohol (CA) and sinapyl alcohol (SA), was attempted with recombinant cationic cell wall-bound peroxidase (rCWPO-C) and horseradish peroxidase (HRP) in measurement cells of a quartz crystal mi-crobalance with dissipation (QCM-D). Hardwood cellulose nanofibers were anchored; hemicelluloses, xylan, partially acetylated xylan (AcXY), galactoglucomannan, and xyloglucan, and the enzymes were subsequently adsorbed onto the QCM-D sensor surface, enabling fabrication of artificial polysaccharide matrices. The largest amount of rCWPO-C is found to be adsorbed onto AcXY among all the polysaccharides, which affords the largest amount and size of spherical dehydrogenation polymers (DHPs) from both CA and SA. In contrast, no DHP and a small amount of DHPs are formed from SA and CA, respectively, by HRP catalysis in all of the polysaccharide matrices. This study demonstrates important functions of a real tree-derived peroxidase, rCWPO-C, and AcXY for hardwood lignification.
Rights: © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 栞

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