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A Basic Study of the Effects of Mulberry Leaf Administration to Mice on Gut Microbiota and Metabolites
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| Title: | A Basic Study of the Effects of Mulberry Leaf Administration to Mice on Gut Microbiota and Metabolites |
| Other Titles: | マウスへの桑の葉投与が腸内細菌叢と代謝物に及ぼす影響に関する基礎研究 |
| Authors: | 甘, 莉 Browse this author |
| Issue Date: | 25-Dec-2023 |
| Publisher: | Hokkaido University |
| Abstract: | Mulberry leaves containα glucosidase inhibitors, which have hypoglycemic effects and are considered functional foods. However, few reports have covered the effects of mulberry leaf components on normal gut microbiota and gut metabolites. Herein, gut microbiota analysis and NMR based metabolomics were performed on the feces of mulberry leaf powder (MLP) treated mice to determine the effects of long term MLP consumption. Gut microbiota in the mouse were analyzed using 16S rRNA gene sequencing, and no significant differences were revealed in the diversity and community structure of the gut microbiota in the C57BL/6 mice with or without MLP supplementation. Thirty nine metabolites were identified via 1 H NMR analysis, and carbohydrates and amino acids were significantly ( p < 0.01 0.05) altered upon MLP treatment. In the MLP treated group, there was a marked increase and decrease in maltose and glucose concentrations, respectively, possibly due to the degradation inhibitory activity of oligosaccharides. After 5 weeks, all ami no acid concentrations decreased. Furthermore, despite clear fluctuations in fecal saccharide concentrations, short chain fatty acid production via intestinal bacterial metabolism was not strongly affected. This study provides the knowledge that MLP administration can alter the gut metabolites without affecting the normal gut microbiota, which is useful for considering MLP as a healthy food source. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第15675号 |
| Degree Level: | 博士 |
| Degree Discipline: | ソフトマター科学 |
| Examination Committee Members: | (主査) 教授 相沢 智康, 教授 芳賀 永, 教授 中村 公則 |
| Degree Affiliation: | 生命科学院（ソフトマター専攻） |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/91221 |
| Appears in Collections: | 課程博士 (Doctorate by way of Advanced Course) > 生命科学院(Graduate School of Life Science)
学位論文 (Theses) > 博士 （ソフトマター科学）
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