|
Hokkaido University Collection of Scholarly and Academic Papers >
Institute of Low Temperature Science >
Peer-reviewed Journal Articles, etc >
Structural Characterization of the Chlorophyllide a Oxygenase (CAO) Enzyme Through an In Silico Approach
| Title: | Structural Characterization of the Chlorophyllide a Oxygenase (CAO) Enzyme Through an In Silico Approach |
| Authors: | Dey, Debayan Browse this author | | Tanaka, Ryouichi Browse this author →KAKEN DB | | Ito, Hisashi Browse this author |
| Keywords: | Chlorophyll b biosynthesis | | Chlorophyllide a oxygenase | | Micromonas pusilla | | Computational prediction | | Molecular docking |
| Issue Date: | 3-Mar-2023 |
| Publisher: | Springer |
| Journal Title: | Journal of molecular evolution |
| Volume: | 91 |
| Start Page: | 225 |
| End Page: | 235 |
| Publisher DOI: | 10.1007/s00239-023-10100-9 |
| Abstract: | Chlorophyllide a oxygenase (CAO) is responsible for converting chlorophyll a to chlorophyll b in a two-step oxygenation reaction. CAO belongs to the family of Rieske-mononuclear iron oxygenases. Although the structure and reaction mechanism of other Rieske monooxygenases have been described, a member of plant Rieske non-heme iron-dependent monooxygenase has not been structurally characterized. The enzymes in this family usually form a trimeric structure and electrons are transferred between the non-heme iron site and the Rieske center of the adjoining subunits. CAO is supposed to form a similar structural arrangement. However, in Mamiellales such as Micromonas and Ostreococcus, CAO is encoded by two genes where non-heme iron site and Rieske cluster localize on the distinct polypeptides. It is not clear if they can form a similar structural organization to achieve the enzymatic activity. In this study, the tertiary structures of CAO from the model plant Arabidopsis thaliana and the Prasinophyte Micromonas pusilla were predicted by deep learning-based methods, followed by energy minimization and subsequent stereochemical quality assessment of the predicted models. Furthermore, the chlorophyll a binding cavity and the interaction of ferredoxin, which is the electron donor, on the surface of Micromonas CAO were predicted. The electron transfer pathway was predicted in Micromonas CAO and the overall structure of the CAO active site was conserved even though it forms a heterodimeric complex. The structures presented in this study will serve as a basis for understanding the reaction mechanism and regulation of the plant monooxygenase family to which CAO belongs. |
| Rights: | This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s00239-023-10100-9 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/91246 |
| Appears in Collections: | 低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 伊藤 寿
|
